首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白M(APOM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOM含量。APOM是一个研究靶点。其在脂质代谢、炎症反应等生理过程中可能发挥重要作用。研究机制方面，聚焦于其对脂蛋白代谢的影响、与细胞信号通路的交互等，旨在揭示其在疾病发生发展中的角色，为相关疾病防治药物研发提供方向。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可广泛应用于小鼠模型中脂代谢机制研究、心血管疾病动物模型评估、肝脏功能调控等基础科研领域，为探索APOM在脂蛋白代谢及相关病理生理过程中的作用提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apom ELISA kit; Ng20Apolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit
缩写：APOM
Uniprot No.：Q9Z1R3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 86-98 | |||||
| 1:8000 | Average % | 100 | ||||
| Range % | 96-107 | |||||
回收率：
| The recovery of mouse APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-99 | ||||
| EDTA plasma (n=4) | 99 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5032.5692.5362.369502.2782.2072.2432.076251.8451.8641.8551.68812.51.3171.3291.3231.1566.250.8610.8990.8800.7133.120.5820.5920.5870.4201.560.4120.4260.4190.25200.1680.1660.167 | ||||||
货期：3-5 working days
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