首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠淀粉样βA4蛋白(APP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APP含量。APP（淀粉样前体蛋白）是备受关注的重要靶点。其背景在于它和阿尔茨海默病密切相关。研究机制上，APP经一系列酶切产生Aβ，异常聚集形成淀粉样斑块，引发神经毒性等病理变化，因此针对APP的干预成为治疗相关疾病的研究方向。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，本试剂盒采用高特异性抗体配对，可在4小时内完成检测流程，适用于大鼠疾病模型构建、神经退行性机制探索、药物干预效果评价等科研场景，尤其适合脑组织、外周体液样本中APP蛋白动态变化的定量追踪。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：App; A4; AD1; Amyloid-beta precursor protein; ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta; A4 precursor protein; Amyloid-beta A4 protein; Amyloidogenic glycoprotein; AG
缩写：APP
Uniprot No.：P08592
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat APP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-107 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-95 | |||||
回收率：
| The recovery of rat APP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.5572.5202.5392.4295001.9611.8761.9191.8092501.1971.2041.2011.0911250.7480.8090.7790.66962.50.4360.4460.4410.33131.20.2780.2590.2690.15915.60.2060.2090.2080.09800.1120.1070.110 | ||||||
货期：3-5 working days
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