首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠载脂蛋白M(APOM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOM含量。APOM为一种载脂蛋白，在脂代谢、炎症反应等生理过程中发挥重要作用。研究机制主要聚焦于其与高密度脂蛋白（HDL）结合，参与胆固醇逆向转运等过程，还可能和动脉粥样硬化、神经退行性疾病等发病机制存在关联，是潜在治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于探索APOM在动脉粥样硬化、糖尿病并发症等病理过程中的生物学功能，也可用于评估药物干预或基因调控对APOM表达水平的影响。为研究脂质代谢机制、建立心血管疾病动物模型提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ApomApolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit; Protein Px ELISA kit
缩写：APOM
Uniprot No.：P14630
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:200 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:400 | Average % | 90 | |||||
| Range % | 85-96 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 88-94 | ||||||
| 1:1600 | Average % | 100 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-94 | |||||
| EDTA plasma (n=4) | 95 | 90-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1002.4542.4602.4572.319502.0452.0322.0391.901251.4111.4821.4471.30912.50.8310.9010.8660.7286.250.6310.6250.6280.4903.120.3790.4080.3940.2561.560.2770.2720.2750.13700.1350.1400.138 | |||||||
货期：3-5 working days
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