| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | U2AF65 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human U2AF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于U2AF2抗体的3篇参考文献，包含文献名称、作者及摘要概括：
1. **文献名称**：*"U2AF2 mutations alter splice site recognition in hematologic malignancies"*
**作者**：Smith A, et al.
**摘要**：研究通过U2AF2抗体进行免疫沉淀和测序，揭示了U2AF2在骨髓增生异常综合征中的突变导致剪接位点选择异常，促进疾病进展。
2. **文献名称**：*"Structural insights into U2AF2 function in mRNA splicing"*
**作者**：Zhang Y, et al.
**摘要**：利用U2AF2特异性抗体进行结构生物学分析，阐明U2AF2与RNA结合域的相互作用机制及其在剪接体组装中的关键作用。
3. **文献名称**：*"U2AF2 as a biomarker in lung adenocarcinoma: antibody-based detection and clinical correlations"*
**作者**：Chen L, et al.
**摘要**：开发高特异性U2AF2单克隆抗体，发现其在肺癌组织中高表达，与患者预后不良相关，提示其作为潜在诊断标志物。
(注：以上文献为示例，实际引用时需根据具体研究补充真实来源。)
The U2AF2 antibody targets the U2 small nuclear RNA auxiliary factor 2 (U2AF2), a critical component of the spliceosome machinery responsible for pre-mRNA splicing. U2AF2. also known as U2AF65. binds to the poly-pyrimidine tract near the 3' splice site of introns, working in tandem with U2AF1 (U2AF35) to recruit the U2 snRNP and ensure accurate recognition of splice sites. This protein is essential for constitutive and alternative splicing, regulating gene expression diversity in eukaryotic cells.
Antibodies against U2AF2 are widely used in molecular biology to study spliceosome assembly, splicing mechanisms, and their dysregulation in diseases. Aberrant splicing linked to U2AF2 mutations or altered expression has been implicated in cancers, neurodegenerative disorders, and myelodysplastic syndromes. Researchers employ U2AF2 antibodies in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze its expression, localization, and interactions with other splicing factors. These tools have also advanced the understanding of how splicing errors contribute to pathogenesis, offering potential diagnostic or therapeutic targets. Commercial U2AF2 antibodies are typically validated for specificity across human, mouse, and rat models, supporting translational research in RNA biology and disease mechanisms.
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