首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠水通道蛋白2(AQP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的AQP2含量。AQP2即水通道蛋白2，是一种位于肾脏集合管主细胞的膜蛋白，在尿液浓缩过程中起关键作用。其表达和功能异常与多种水代谢紊乱疾病相关。目前研究聚焦于其调节机制，如受抗利尿激素调控等，有望为相关疾病治疗提供新思路。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于研究肾脏水代谢调控机制、泌尿系统疾病模型建立、药物干预对水通道蛋白的影响等科研领域，例如在高血压或糖尿病肾病动物模型中探讨AQP - 2的动态变化规律，或评估利尿剂等药物对肾脏水重吸收功能的调节作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aqp2; Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
缩写：AQP2
Uniprot No.：P34080
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AQP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-98 | |||||
回收率：
| The recovery of rat AQP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 95-106 | ||||
| EDTA plasma (n=4) | 94 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.5612.7842.6732.4962001.8231.8271.8251.6481001.1561.1671.1620.985500.7540.7890.7720.595250.4650.4870.4760.29912.50.3370.3640.3510.1746.250.2640.2750.2700.09300.1750.1790.177 | ||||||
货期：3-5 working days
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