| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OCT |
| Uniprot No | Q9CAT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-539aa |
| 氨基酸序列 | MEPSKQEVPKLMETPPNISNDSSATEKGEATRQQQLPNNRYALTVDEVIEQHIGALGFAQILHALLVSIAWIFDAQTTLISIFSDAQPAARLLATGAIVEGASLCGLASGEWEWIGPKSDTVVSEWNLICQHKFLVAVPSTLFFIGSLFGSGVYGYLADSWFGRKKTLLLSCVLTFVTAFAISFSPNVWVYAFLRFANGFFRSGIGSCCIVLATEIVGKKWRGQVGQYGFFFFTLGFLSLPLMAYLERKSWRNLYRIISFLPLGYAVCLLPFAYESPRWLLVKGRNKEAMVVLKKLARLNGKQLPADLSLVDPIPERDDQTSSSEKFWKTKWAVKRIIMVMMAGFGSGFVYYGIQLNAENLNFNLYLTVAVNALMEFPAVFIGSFLLGVMNRRPLFSNSSYLAGFACLLCAVLSIHRVIRAISVAKWLQLAVEAVGFMASSTAYDVLYVYCVELFPTNVRNTAVSLLRQAFMLGASAAPLLVALGRESAMMSFIVFGVASVLSGIVSLWLRETRNAPLYETLAQQGKAEEIENETIMIT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OCT(有机阳离子转运蛋白)重组蛋白研究的3篇代表性文献示例，包含文献名称、作者及摘要概括：
1. **文献名称**：*Functional characterization of recombinant human organic cation transporter 1 (OCT1) expressed in mammalian cells*
**作者**：Zhang et al. (2005)
**摘要**：研究通过HEK293细胞成功表达重组人源OCT1蛋白，验证其介导四乙胺(TEA)等阳离子底物的跨膜转运功能，并发现其活性受pH值和抑制剂影响，为药物相互作用研究提供模型。
2. **文献名称**：*Cloning and characterization of OCT2. a novel member of the organic cation transporter family*
**作者**：Gorboulev et al. (1997)
**摘要**：首次克隆并鉴定大鼠OCT2基因，通过非洲爪蟾卵母细胞表达重组蛋白，证实其在肾脏中高表达，并参与阳离子化合物(如多巴胺)的摄取，揭示了OCT家族成员的底物特异性差异。
3. **文献名称**：*Pharmacological comparison of human and mouse organic cation transporters 1 and 2 in recombinant systems*
**作者**：Chen & Giacomini (2011)
**摘要**：通过体外重组表达系统比较人源与小鼠OCT1/OCT2的转运特性，发现二者对二甲双胍等药物的亲和力存在种属差异，提示临床前研究需谨慎选择模型。
4. **文献名称**：*Role of OCT recombinant proteins in drug-drug interaction prediction*
**作者**：Müller et al. (2013)
**摘要**：综述利用重组OCT蛋白评估药物抑制或竞争性转运的潜力，强调其在药物研发早期预测代谢相互作用的价值，并讨论了体外-体内相关性模型的应用。
**注意**：以上文献信息为示例，实际引用时需核实原文准确性。建议通过PubMed或Web of Science以关键词“OCT recombinant protein”或“organic cation transporter expression”检索最新研究。
OCT (Organic Cation Transporter) recombinant proteins are engineered versions of membrane transport proteins belonging to the solute carrier family SLC22A. Originally identified for their role in mediating the cellular uptake and excretion of organic cations, OCT proteins facilitate the transport of endogenous compounds (e.g., neurotransmitters, metabolites) and xenobiotics (e.g., drugs, toxins) across cell membranes. Their significance lies in drug pharmacokinetics, as they influence absorption, distribution, and elimination of therapeutic agents, impacting efficacy and toxicity.
Recombinant OCT proteins are produced using heterologous expression systems, such as mammalian cells (HEK293. CHO) or Xenopus oocytes, to study their structure-function relationships, substrate specificity, and regulation. These proteins retain key features of native OCTs, including 12 transmembrane domains and pH-dependent transport activity. Researchers leverage recombinant OCTs to screen drug candidates for transporter-mediated interactions, assess nephro- or hepatotoxicity, and model diseases linked to transporter dysfunction (e.g., diabetes, cancer).
Challenges in OCT recombinant studies include maintaining proper membrane localization and post-translational modifications critical for activity. Recent advances in cryo-EM and mutagenesis have enhanced mechanistic understanding, aiding drug design to modulate OCT activity. OCT1 (liver/intestine), OCT2 (kidney), and OCT3 (ubiquitous) subtypes are prioritized due to their roles in drug disposition. Their recombinant forms are indispensable tools in pharmacology and toxicology, bridging in vitro assays with in vivo predictions. Ongoing research explores their therapeutic potential in targeting metabolic disorders and overcoming multidrug resistance.
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