| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSTF1 |
| Uniprot No | Q92882 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-214aa |
| 氨基酸序列 | SKPPPKPVK PGQVKVFRAL YTFEPRTPDE LYFEEGDIIY ITDMSDTNWW KGTSKGRTGL IPSNYVAEQA ESIDNPLHEA AKRGNLSWLR ECLDNRVGVN GLDKAGSTAL YWACHGGHKD IVEMLFTQPN IELNQQNKLG DTALHAAAWK GYADIVQLLL AKGARTDLRN IEKKLAFDMA TNAACASLLK KKQGTDAVRT LSNAEDYLDD EDSD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSTF1重组蛋白的3篇假设参考文献示例(注：内容为示例，实际文献需查询数据库)：
1. **文献名称**：*Recombinant OSTF1 Enhances Osteoclastogenesis via NF-κB Signaling*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达OSTF1重组蛋白，证实其通过激活NF-κB通路促进破骨细胞分化，为骨吸收疾病提供潜在机制。
2. **文献名称**：*Expression and Purification of Functional OSTF1 Protein in Mammalian Cells*
**作者**：Zhang L, et al.
**摘要**：利用HEK293细胞成功表达具有生物活性的OSTF1重组蛋白，并验证其在调控骨代谢相关基因中的功能。
3. **文献名称**：*OSTF1 Recombinant Protein Attenuates Bone Loss in Ovariectomized Mice*
**作者**：Johnson R, et al.
**摘要**：在小鼠骨质疏松模型中，注射OSTF1重组蛋白显著抑制骨密度下降，提示其治疗骨疾病的潜力。
建议通过 **PubMed** 或 **Google Scholar** 检索实际文献(关键词：OSTF1 recombinant protein, osteoclast, bone metabolism)。
**Background of Recombinant OSTF1 Protein**
Osteoclast-stimulating factor 1 (OSTF1), also known as OSF-1 or SH3 domain-containing protein 2 (SH3P2), is a signaling adaptor protein involved in regulating cellular processes such as cytoskeletal reorganization, cell motility, and bone resorption. It was initially identified for its role in osteoclast activation, where it enhances osteolytic activity by interacting with signaling pathways like Src kinase and RANK (receptor activator of nuclear factor kappa-β). Structurally, OSTF1 contains an N-terminal SH3 domain that mediates protein-protein interactions and a C-terminal region critical for its function in intracellular signaling.
Recombinant OSTF1 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and activity. This engineered protein serves as a valuable tool for studying OSTF1’s molecular mechanisms in physiological and pathological contexts. For example, it has been used in *in vitro* assays to investigate its role in cancer metastasis, where OSTF1 overexpression correlates with increased cell migration and invasion. Additionally, recombinant OSTF1 aids in exploring bone-related disorders, such as osteoporosis, by mimicking or blocking endogenous OSTF1 activity in osteoclast cultures.
Research also highlights OSTF1’s dual roles in different cancers, acting as either a promoter or suppressor of tumor progression depending on the cellular context. Its recombinant form enables detailed structural analyses, antibody development, and drug screening for therapeutic targeting. Overall, recombinant OSTF1 protein provides a versatile platform for advancing our understanding of bone biology, cancer metastasis, and potential therapeutic interventions.
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