首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠精氨酸酶1(ARG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ARG1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，常用于大鼠模型中肝脏损伤、炎症反应或代谢异常的机制研究；适用于科研领域中对大鼠ARG1蛋白水平的精准分析，例如评估药物干预、病理模型或基因编辑对肝脏代谢通路的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit
缩写：ARG1
Uniprot No.：P07824
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-95 | |||||
回收率：
| The recovery of rat ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 95-109 | ||||
| EDTA plasma (n=4) | 101 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4462.3452.3962.225101.9921.9141.9531.78251.2551.3261.2911.1202.50.7160.7320.7240.5531.250.4200.4330.4270.2560.6250.3210.3380.3300.1590.3120.2390.2470.2430.07200.1690.1730.171 | ||||||
货期：3-5 working days
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