| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OXSM |
| Uniprot No | Q9NWU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-459aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSKRKFFGTVPISRLHRRVVITGIGLVTP LGVGTHLVWDRLIGGESGIVSLVGEEYKSIPCSVAAYVPRGSDEGQFNEQ NFVSKSDIKSMSSPTIMAIGAAELAMKDSGWHPQSEADQVATGVAIGMGM IPLEVVSETALNFQTKGYNKVSPFFVPKILVNMAAGQVSIRYKLKGPNHA VSTACTTGAHAVGDSFRFIAHGDADVMVAGGTDSCISPLSLAGFSRARAL STNSDPKLACRPFHPKRDGFVMGEGAAVLVLEEYEHAVQRRARIYAEVLG YGLSGDAGHITAPDPEGEGALRCMAAALKDAGVQPEEISYINAHATSTPL GDAAENKAIKHLFKDHAYALAVSSTKGATGHLLGAAGAVEAAFTTLACYY QKLPPTLNLDCSEPEFDLNYVPLKAQEWKTEKRFIGLTNSFGFGGTNATL CIAGL |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OXSM重组蛋白的3篇虚构参考文献示例，基于常见研究场景构建：
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1. **文献名称**：*Heterologous Expression and Enzymatic Characterization of Recombinant Human OXSM in E. coli*
**作者**：Zhang, L.; Wang, Y.; Chen, T.
**摘要**：本研究成功将人源OXSM基因克隆至大肠杆菌表达系统，纯化获得高纯度重组蛋白。酶活实验证实其催化3-oxoacyl-ACP底物转化为酮体的功能，为后续药物筛选提供基础。
2. **文献名称**：*Crystal Structure of Mitochondrial OXSM Reveals a Novel Substrate-Binding Mechanism*
**作者**：Smith, J.R.; Tanaka, K.; Müller, R.
**摘要**：通过X射线晶体学解析了OXSM的2.8Å分辨率结构，发现其活性中心独特的疏水口袋结构，结合分子动力学模拟阐明了底物识别与催化循环的分子机制。
3. **文献名称**：*OXSM Deficiency Disrupts Hepatic Ketogenesis in a Mouse Model*
**作者**：Brown, A.E.; Silva, M.; Gupta, S.
**摘要**：构建OXSM基因敲除小鼠模型，发现肝脏酮体水平显著降低，导致能量代谢紊乱，证实OXSM在脂代谢中的关键作用，为相关代谢疾病研究提供依据。
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这些示例涵盖了重组蛋白的表达、结构解析及功能研究，符合常见文献主题。实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
OXSM (3-Oxoacyl-ACP Synthase, Mitochondrial) is a key enzyme involved in mitochondrial fatty acid synthesis (mtFAS), a conserved pathway critical for cellular metabolism. As a member of the 3-oxoacyl-ACP synthase family, it catalyzes the condensation step in fatty acid elongation, specifically in mitochondria, by combining malonyl-ACP with acyl-ACP substrates. This process generates 3-ketoacyl-ACP intermediates essential for producing lipoic acid—a cofactor required for mitochondrial enzyme complexes like pyruvate dehydrogenase and α-ketoglutarate dehydrogenase. Unlike bacterial homologs (e.g., FabH), OXSM is nuclear-encoded, contains a mitochondrial targeting sequence, and operates in eukaryotic mitochondrial ACP-dependent pathways.
Recombinant OXSM proteins are engineered for structural and functional studies to elucidate mtFAS mechanisms. They are typically expressed in *E. coli* or yeast systems, purified via affinity tags, and used to analyze substrate specificity, enzymatic kinetics, and interactions with other mtFAS components. Research highlights OXSM’s role in maintaining mitochondrial lipid homeostasis and its link to human diseases, including metabolic disorders and neurodegenerative conditions. For example, OXSM dysfunction may impair lipoylation, disrupting energy metabolism and contributing to pathologies like Leigh syndrome.
Additionally, recombinant OXSM serves as a tool for drug discovery, particularly in targeting bacterial FAS pathways (divergent from mammalian systems) for antibiotic development. Its mitochondrial specificity also makes it a potential biomarker for metabolic syndrome or cancer, where altered lipid metabolism is prevalent. Ongoing studies focus on crystallizing OXSM to map active sites and design inhibitors, while genetic models (e.g., OXSM knockout mice) help validate its physiological roles. Overall, recombinant OXSM bridges fundamental biochemistry with translational applications in health and disease.
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