| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human BRPF3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BRPF3抗体的3篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*BRPF3 is a novel histone acetyltransferase reader critical for hematopoietic stem cell maintenance*
**作者**：Klein BJ, et al.
**摘要**：本研究揭示了BRPF3作为MOZ/MORF组蛋白乙酰转移酶复合体的关键调控因子。通过制备特异性BRPF3抗体，作者验证了其在造血干细胞中的表达，并证明其通过识别乙酰化组蛋白维持干细胞功能。
2. **文献名称**：*Development and validation of a polyclonal antibody for detecting BRPF3 in human cancers*
**作者**：Wang Y, et al.
**摘要**：该研究报道了一种针对人源BRPF3蛋白的多克隆抗体的开发过程，通过重组蛋白免疫兔制备抗体，并利用Western blot和免疫组化验证其特异性。结果显示BRPF3在多种肿瘤组织中高表达。
3. **文献名称**：*BRPF3 modulates neural crest cell migration via chromatin remodeling during embryonic development*
**作者**：Chen L, et al.
**摘要**：研究利用BRPF3抗体进行免疫荧光和染色质免疫沉淀(ChIP)，发现BRPF3通过调控染色质重塑相关基因(如Hoxa2)的表达，影响神经嵴细胞的迁移及胚胎发育。
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**注**：上述文献为示例性质，具体内容需根据实际发表的论文调整。建议通过PubMed或Google Scholar以关键词“BRPF3 antibody”或“BRPF3 immunohistochemistry”检索最新研究。
The BRPF3 (Bromodomain and PHD Finger-containing Protein 3) antibody is a tool used to detect and study the BRPF3 protein, a member of the BRPF family involved in epigenetic regulation. BRPF3 acts as a regulatory subunit of histone acetyltransferase (HAT) complexes, such as the MOZ/MORF complex, which modulate chromatin structure and gene expression by acetylating histones. This protein contains conserved bromodomains and PHD fingers that facilitate interactions with acetylated histones or other chromatin-associated proteins, playing roles in transcriptional activation, cellular differentiation, and development.
BRPF3 antibodies are widely utilized in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate BRPF3’s expression, localization, and molecular interactions. These antibodies help elucidate BRPF3’s involvement in diseases, particularly cancers (e.g., leukemia) and neurodevelopmental disorders, where dysregulation of epigenetic modifiers is common. Validated for specificity, BRPF3 antibodies are often tested in knockout cell lines or tissues to confirm minimal cross-reactivity with related family members (e.g., BRPF1/2). Research using these antibodies has highlighted BRPF3’s role in maintaining stem cell pluripotency and regulating developmental pathways. Commercial BRPF3 antibodies are typically raised against epitopes in the N-terminal or central regions, with reactivity across human, mouse, and rat samples. Their applications extend to both basic research and therapeutic target validation in epigenetics-focused studies.
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