首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠脑源性神经营养因子(BDNF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的BDNF含量。BDNF即脑源性神经营养因子，在神经系统发育和维持中起重要作用。其背景在于它参与神经元存活、分化等过程。研究机制方面，主要通过与TrkB受体结合，激活相关信号通路，进而调节神经突触可塑性、神经元生长及神经递质释放等。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可通过探究中枢神经系统疾病模型（如阿尔茨海默病、抑郁症）中BDNF的动态变化，评估神经保护药物作用效果，或分析体外培养神经细胞分泌功能。试剂盒兼容多种生物样本类型，为神经发育、损伤修复及信号通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BdnfBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
缩写：BDNF
Uniprot No.：P21237
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 99-109 | |||||
回收率：
| The recovery of mouse BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-101 | ||||
| EDTA plasma (n=4) | 88 | 81-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.4202.3552.3882.251 5001.7121.6851.6991.562 2500.9840.9940.9890.852 1250.5870.5680.5780.441 62.50.3930.3770.3850.248 31.20.2230.2360.2300.093 7.80.1500.1600.1550.018 00.1340.1390.137 | ||||||
货期：3-5 working days
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