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产品描述：犬骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BGLAP含量。BGLAP即骨钙素，是骨组织中最为丰富的非胶原蛋白。其背景上，与骨骼生长、代谢密切相关。研究机制方面，它可调节骨代谢平衡，还在能量代谢、生殖等过程中发挥作用，目前围绕它在疾病诊治中的潜在价值是研究热点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其应用于犬类骨骼发育研究、骨质疏松模型构建、骨代谢相关药物筛选，以及营养干预对骨组织影响等基础研究领域，为犬科动物骨生物学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：BGLAP
Uniprot No.：P81455
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 87-102 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of dog OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-96 | ||||
| EDTA plasma (n=4) | 102 | 95-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected203.1053.0043.0552.953102.6542.6742.6642.56251.8781.8891.8841.7822.51.0361.1051.0710.9691.250.4680.5000.4840.3820.6250.2610.2730.2670.1650.3120.1580.1640.1610.05900.1030.1000.102 | ||||||
货期：3-5 working days
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