| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OST; WBP1; AGER1; CDG1R; OST48; OKSWcl45 |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DDOST |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDOST抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*DDOST as a novel diagnostic and prognostic biomarker for hepatocellular carcinoma*
**作者**：Li Y, et al.
**摘要**：该研究通过免疫组化技术使用DDOST抗体，发现DDOST在肝细胞癌(HCC)组织中显著高表达，并与患者预后不良相关，提示其可能作为HCC的新型生物标志物。
2. **文献名称**：*Autoantibody targeting DDOST in autoimmune encephalitis: clinical implications*
**作者**：Wang Q, et al.
**摘要**：研究发现部分自身免疫性脑炎患者血清中存在抗DDOST抗体，通过ELISA和蛋白质印迹法验证其特异性，提示DDOST可能参与自身免疫反应机制。
3. **文献名称**：*DDOST regulates protein N-glycosylation and promotes tumor invasion in breast cancer*
**作者**：Zhang H, et al.
**摘要**：利用DDOST抗体敲低实验证实，DDOST通过调控蛋白质N-糖基化过程促进乳腺癌细胞侵袭，为靶向糖基化通路治疗提供依据。
(注：以上文献为示例性内容，实际引用需以具体论文数据为准。)
DDOST (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) is a critical enzyme in the oligosaccharyltransferase (OST) complex, which mediates the central step of N-linked glycosylation in the endoplasmic reticulum. This post-translational modification is essential for protein folding, quality control, and cellular trafficking. DDOST, also known as ribophorin II, anchors the catalytic STT3 subunit to the ER membrane, ensuring proper recognition and transfer of oligosaccharides to nascent polypeptides.
Antibodies targeting DDOST are valuable tools for studying N-glycosylation mechanisms and associated diseases. Mutations in DDOST have been linked to congenital disorders of glycosylation (CDGs), characterized by multisystemic abnormalities. Researchers use DDOST antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions within the OST complex.
Additionally, DDOST dysregulation has been implicated in cancer, neurodegeneration, and immune disorders, making these antibodies relevant for biomarker discovery and therapeutic development. Their specificity enables differentiation between functional OST isoforms and aids in elucidating tissue-specific glycosylation pathways. Overall, DDOST antibodies serve as key reagents for advancing glycobiology research and understanding diseases rooted in protein glycosylation defects.
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