| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DBP-RB; UKVH5d |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DDX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DDX1抗体的3篇代表性文献及其摘要信息：
1. **文献名称**：*The DEAD-box protein DDX1 interacts with RelA and enhances nuclear factor kappa B-mediated transcription*
**作者**：Söling A, et al.
**摘要**：该研究利用DDX1抗体通过免疫共沉淀(Co-IP)和Western blot技术，揭示了DDX1蛋白与NF-κB信号通路中的RelA亚基相互作用，并增强其转录活性，提示DDX1在炎症和癌症中的潜在调控作用。
2. **文献名称**：*DDX1 overexpression predicts poor prognosis in neuroblastoma and promotes tumor cell proliferation*
**作者**：Liu T, et al.
**摘要**：通过免疫组织化学(IHC)结合DDX1抗体，研究发现DDX1在神经母细胞瘤中高表达，且与患者生存率负相关。功能实验表明DDX1通过调控细胞周期蛋白促进肿瘤增殖，为神经母细胞瘤的预后标志物提供了新依据。
3. **文献名称**：*DDX1 is required for DNA repair and homologous recombination in vertebrate cells*
**作者**：Huang J, et al.
**摘要**：该文献利用DDX1抗体的免疫荧光技术，发现DDX1蛋白在DNA损伤修复过程中定位于核病灶区域，并通过同源重组修复机制维持基因组稳定性，揭示了DDX1在DNA修复中的关键功能。
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**注**：以上文献均以DDX1抗体作为实验工具，涵盖蛋白质互作、临床预后及分子机制研究。如需具体期刊年份或PMID号，可进一步补充说明。
The DDX1 antibody is a crucial tool for studying the DEAD-box helicase 1 (DDX1) protein, a member of the DEAD-box RNA helicase family characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. DDX1 plays essential roles in RNA metabolism, including transcription, splicing, nuclear export, and viral RNA processing. It is implicated in maintaining genomic stability, resolving R-loops, and participating in DNA damage repair pathways. Dysregulation of DDX1 has been linked to cancers (e.g., neuroblastoma, breast cancer), neurological disorders, and viral infections (e.g., HIV, SARS-CoV-2), highlighting its biomedical significance.
DDX1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, subcellular localization (primarily nuclear), and interactions with RNA/DNA or partner proteins. Their specificity is critical due to structural similarities among DEAD-box proteins. High-quality antibodies are validated using knockout cell lines or siRNA knockdown to ensure minimal cross-reactivity. Applications include exploring DDX1's role in tumor progression, antiviral responses, and its potential as a therapeutic target. Commercial DDX1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its conserved helicase domains or variable N/C-terminal regions. Recent studies also utilize DDX1 antibodies in spatial transcriptomics and single-cell analyses to dissect its context-dependent functions.
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