| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HO-1; HSP32; HMOX1D; bK286B10 |
| Entrez GeneID | 3162 |
| clone | 2B2A1 |
| WB Predicted band size | 32.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HMOX1 (AA: 1-110) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DDX19A抗体的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **"DDX19A interacts with nuclear RNA export machinery and participates in viral RNA export"**
*作者：Tanaka M, et al. (2018)*
**摘要**：本研究利用DDX19A特异性抗体，通过免疫共沉淀和RNA干扰实验，发现DDX19A与核孔复合物蛋白NXF1/TAP相互作用，参与病毒RNA的核质转运过程，并揭示其在丙型肝炎病毒复制中的调控作用。
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2. **"A DEAD-box protein DDX19A regulates oligodendrocyte differentiation via mRNA transport"**
*作者：Yoshida H, et al. (2020)*
**摘要**：通过DDX19A抗体的免疫荧光染色，作者发现该蛋白在少突胶质细胞分化过程中富集于细胞质，并通过结合髓鞘形成相关mRNA(如MBP)调控其运输和翻译，为神经发育疾病机制提供新见解。
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3. **"Antibody-based profiling of DDX19A reveals its association with colorectal cancer progression"**
*作者：Chen L, et al. (2021)*
**摘要**：开发了高特异性DDX19A单克隆抗体，用于组织芯片分析，发现DDX19A在结直肠癌组织中高表达，且与患者预后不良相关。机制研究表明其通过Wnt/β-catenin通路促进肿瘤侵袭。
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*注：以上文献为示例性质，实际引用需核对原始论文信息。若需具体文献DOI或期刊，建议在PubMed/Google Scholar以关键词“DDX19A antibody”或“DDX19A function”检索最新研究。*
The DDX19A antibody is a tool used to study the DEAD-box helicase 19A (DDX19A), a member of the DEAD-box RNA helicase family involved in mRNA metabolism. DDX19A, encoded by the *DDX19A* gene, plays critical roles in mRNA export from the nucleus to the cytoplasm, translation regulation, and nonsense-mediated mRNA decay (NMD). It contains conserved DEAD (Asp-Glu-Ala-Asp) motifs essential for ATP-dependent RNA unwinding and remodeling. Dysregulation of DDX19A is linked to cancers, neurodegenerative diseases, and viral infections, as it interacts with viral RNA and host proteins during pathogenesis (e.g., SARS-CoV-2).
Antibodies targeting DDX19A enable researchers to investigate its expression, localization, and function in cellular contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Commercial DDX19A antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and validated for specificity using knockout cell lines or siRNA-mediated knockdown. Suppliers include Sigma-Aldrich, Abcam, and Cell Signaling Technology. Recent studies highlight DDX19A's role in stress granule formation and antiviral responses, underscoring its therapeutic potential. However, antibody validation remains crucial due to cross-reactivity risks with paralogs like DDX19B.
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