| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GIP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SMS(鞘磷脂合成酶，Sphingomyelin Synthase)抗体的3篇示例参考文献，内容基于典型研究方向模拟整理，供参考：
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### 1. **"Monoclonal antibody development for human sphingomyelin synthase 1 (SMS1) and its subcellular localization analysis"**
**作者**: Y. Takahashi, K. Shimizu, et al.
**摘要**: 本研究报道了针对人源SMS1蛋白的单克隆抗体的开发。通过重组蛋白免疫小鼠获得抗体，验证其特异性后，应用于免疫荧光和Western blot分析，揭示SMS1主要定位于高尔基体，并参与细胞膜鞘脂代谢调控。
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### 2. **"Sphingomyelin synthase 2 (SMS2) deficiency modulates hepatic lipid metabolism via antibody-mediated inhibition studies"**
**作者**: H. Wang, L. Chen, et al.
**摘要**: 文章利用抗SMS2的多克隆抗体，在小鼠模型中研究SMS2对肝脏脂代谢的影响。实验表明，抗体介导的SMS2功能抑制可减少甘油三酯积累，提示SMS2可能成为治疗非酒精性脂肪肝的潜在靶点。
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### 3. **"A novel anti-SMS2 antibody suppresses tumor growth in colorectal cancer models"**
**作者**: M. Rodriguez, S. Gupta, et al.
**摘要**: 该研究开发了一种靶向SMS2的单克隆抗体，并在结直肠癌细胞系及异种移植模型中验证其抗肿瘤效果。结果显示，抗体通过阻断SMS2介导的鞘磷脂合成通路，诱导癌细胞凋亡并抑制增殖。
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### 4. **"Review: Functional roles of sphingomyelin synthases and their antibodies in neurodegenerative diseases"**
**作者**: J. Park & T. Okazaki
**摘要**: 这篇综述总结了SMS1/SMS2在中枢神经系统中的功能，重点探讨了特异性抗体在阿尔茨海默病等神经退行性疾病研究中的应用，包括检测酶表达变化及潜在治疗策略。
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**备注**：以上为模拟生成的参考文献，实际研究中建议通过PubMed、Web of Science等数据库以关键词(如“sphingomyelin synthase antibody”“SMS1/2 inhibitor”)检索最新文献。
**Background of SMS Antibodies**
Sperm Mitochondria-associated Cysteine-rich Protein (SMCP), often referred to as SMS due to historical nomenclature, is a protein predominantly localized in the mitochondria of sperm cells. It plays a critical role in sperm maturation, motility, and structural integrity by stabilizing the mitochondrial sheath during spermatogenesis. SMS antibodies are immunological tools developed to detect and study this protein, aiding in research on male fertility, sperm dysfunction, and reproductive biology.
First identified in the 1990s, SMCP is cysteine-rich, enabling disulfide bond formation crucial for mitochondrial sheath organization. Dysregulation of SMCP expression has been linked to asthenozoospermia (reduced sperm motility) and infertility. SMS antibodies, typically generated in rabbits or mice via recombinant protein immunization, are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize SMCP distribution and expression levels in spermatozoa.
Recent studies also explore SMS antibodies in diagnostic contexts, assessing their potential as biomarkers for male infertility. Additionally, they contribute to understanding evolutionary conservation of sperm-specific proteins across species. Despite their niche application, SMS antibodies remain vital in advancing reproductive medicine and andrology research, bridging molecular insights with clinical outcomes.
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