| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GK001; MSTP041 |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CCDC47 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCDC47抗体的参考文献示例(注：以下为虚构文献，实际引用请查询学术数据库)：
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1. **文献名称**: "CCDC47 modulates endoplasmic reticulum-mitochondria contacts via a specific polyclonal antibody analysis"
**作者**: Chen L, et al.
**摘要**: 本研究利用新开发的CCDC47多克隆抗体，通过免疫荧光和共聚焦显微镜技术，揭示了CCDC47在内质网-线粒体膜接触位点的定位，并证明其参与调控钙离子信号传导和细胞凋亡。
2. **文献名称**: "Development and validation of a CCDC47 monoclonal antibody for immunohistochemical applications in gastrointestinal cancers"
**作者**: Müller S, et al.
**摘要**: 报道了一种高特异性CCDC47单克隆抗体的开发，验证了其在结直肠癌组织中的免疫组化应用，发现CCDC47高表达与患者预后不良相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: "CCDC47 interacts with the protein translocation machinery: Insights from co-immunoprecipitation using a novel antibody"
**作者**: Tanaka K, et al.
**摘要**: 通过新型CCDC47抗体进行免疫共沉淀实验，发现其与Sec61蛋白转运复合物相互作用，证实CCDC47在内质网介导的蛋白质翻译后修饰中起关键作用。
4. **文献名称**: "Antibody-based functional screening identifies CCDC47 as a regulator of autophagosome-lysosome fusion"
**作者**: Gupta R, et al.
**摘要**: 利用CCDC47抗体进行功能筛选实验，发现敲低CCDC47会抑制自噬体-溶酶体融合，表明其在细胞自噬通路中的必要性，并揭示其与LC3蛋白的共定位关系。
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建议通过PubMed、Google Scholar等平台以“CCDC47 antibody”或“CCDC47 function”为关键词检索真实文献。
CCDC47 (Coiled-coil domain-containing protein 47) is a conserved eukaryotic protein primarily localized to the endoplasmic reticulum (ER). It plays a critical role in maintaining ER homeostasis, particularly in calcium ion regulation and protein processing. The protein is characterized by multiple coiled-coil domains, which mediate protein-protein interactions, and a C-terminal ER-retention signal. Studies suggest CCDC47 interacts with ER chaperones like GRP78/BiP, implicating its involvement in protein folding, quality control, and stress response pathways. Dysregulation of CCDC47 has been linked to cellular stress conditions and diseases, including cancer and neurodegenerative disorders.
Antibodies targeting CCDC47 are essential tools for investigating its expression, subcellular localization, and molecular functions. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study tissue-specific expression patterns or alterations under pathological conditions. Several commercial antibodies have been validated for human, mouse, and rat homologs, though species cross-reactivity should be confirmed experimentally. Recent research leverages CCDC47 antibodies to explore its potential as a biomarker in tumor progression or its role in ER-mitochondrial crosstalk. Proper validation, including knockout cell line controls, is recommended to ensure antibody specificity given the protein's ubiquitous expression and structural motifs that may pose epitope challenges.
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