| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDIA3 |
| Uniprot No | P30101 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-505aa |
| 氨基酸序列 | SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL |
| 预测分子量 | 59.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDIA3重组蛋白的3篇文献示例(注：文献为虚拟示例，实际引用需核对真实数据库)：
1. **"Structural insights into PDIA3-mediated disulfide bond formation in MHC class I molecules"**
- **作者**: Lindquist et al.
- **摘要**: 本研究通过重组PDIA3蛋白的晶体结构解析，揭示了其与MHC I类分子相互作用的结构基础，证明PDIA3通过调控二硫键形成参与抗原提呈过程。
2. **"PDIA3 overexpression promotes ovarian cancer progression via ER stress signaling"**
- **作者**: Anttonen et al.
- **摘要**: 利用重组PDIA3蛋白进行体外功能实验，发现其过表达通过激活内质网应激通路增强卵巢癌细胞存活和转移能力，提示其作为治疗靶点的潜力。
3. **"Recombinant PDIA3 modulates platelet activation through thiol-redox mechanisms"**
- **作者**: Turano et al.
- **摘要**: 通过重组PDIA3蛋白研究其在血小板中的功能，发现其通过调控硫醇-氧化还原平衡抑制血小板过度激活，为血栓性疾病提供新干预策略。
4. **"PDIA3 interacts with SARS-CoV-2 spike protein and facilitates viral entry"**
- **作者**: Coppinger et al.
- **摘要**: 重组PDIA3蛋白与新冠病毒刺突蛋白的结合实验表明，PDIA3可能通过促进病毒-宿主膜融合增强感染，为抗病毒药物开发提供线索。
(注：以上文献为假设性概括，实际研究需参考PubMed、Web of Science等数据库。)
**Background of PDIA3 Recombinant Protein**
PDIA3 (Protein Disulfide Isomerase Family A Member 3), also known as ERp57 or GRP58. is a member of the protein disulfide isomerase (PDI) family, which plays a critical role in protein folding and quality control within the endoplasmic reticulum (ER). It facilitates the formation, isomerization, and reduction of disulfide bonds in nascent polypeptides, ensuring proper tertiary structure. Additionally, PDIA3 acts as a chaperone, assisting in the assembly of major histocompatibility complex (MHC) class I molecules by interacting with the peptide-loading complex.
The recombinant PDIA3 protein is produced using genetic engineering techniques, often expressed in bacterial, yeast, or mammalian systems to ensure high purity and functionality. Recombinant expression allows researchers to study PDIA3’s biochemical properties, structural interactions, and involvement in cellular processes such as ER stress response, antigen presentation, and calcium homeostasis. Dysregulation of PDIA3 has been linked to diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s disease), and autoimmune conditions, highlighting its therapeutic and diagnostic potential.
In cancer, PDIA3 overexpression correlates with tumor progression and chemoresistance, while in neurodegeneration, it may mitigate oxidative stress or contribute to pathogenic protein aggregation. Its role in autoimmune diseases involves aberrant immune recognition due to altered post-translational modifications. Recombinant PDIA3 serves as a vital tool for elucidating these mechanisms, screening drug candidates, and developing targeted therapies. Structural studies using recombinant PDIA3 have also advanced understanding of its thioredoxin-like domains and substrate-binding specificity. Overall, PDIA3 recombinant protein remains a key focus in both basic research and translational applications.
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