| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBC2; HHR6A; MRXSN; RAD6A; MRXS30 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human UBE2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是几篇关于UBE2A抗体的虚构参考文献示例，供参考：
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1. **文献名称**：*UBE2A Antibody Validation for Detection of Ubiquitination in Neurodegenerative Models*
**作者**：Chen L, et al.
**摘要**：本研究验证了一种高特异性UBE2A多克隆抗体在阿尔茨海默症细胞模型中的应用。通过免疫印迹和免疫荧光实验，证实该抗体可有效识别UBE2A蛋白，并揭示其在神经元泛素化通路中的调控作用，为神经退行性疾病机制研究提供工具支持。
2. **文献名称**：*Role of UBE2A in DNA Repair: Insights from CRISPR/Cas9 and Antibody-Based Knockdown*
**作者**：Martinez R, et al.
**摘要**：利用UBE2A特异性抗体进行免疫共沉淀(Co-IP)，结合CRISPR/Cas9基因编辑技术，揭示了UBE2A在DNA损伤修复中与BRCA1蛋白的相互作用，表明其作为潜在癌症治疗靶点的可能性。
3. **文献名称**：*Development of a Monoclonal Antibody Against UBE2A for Clinical Pathology Analysis*
**作者**：Tanaka K, et al.
**摘要**：报道了一种新型UBE2A单克隆抗体的开发与表征。该抗体通过ELISA和免疫组化验证，成功应用于肿瘤组织样本检测，显示UBE2A在结直肠癌中表达显著上调，提示其与肿瘤进展的相关性。
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**注**：以上文献为虚构示例，仅用于展示回答格式。实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
The UBE2A antibody is a crucial tool in studying the ubiquitin-conjugating enzyme E2A (UBE2A), a member of the E2 ubiquitin-conjugating enzyme family. UBE2A, also known as RAD6A, plays a pivotal role in the ubiquitin-proteasome system by transferring ubiquitin to target proteins, marking them for degradation or functional modification. This enzyme is involved in critical cellular processes, including DNA damage repair, transcriptional regulation, and cell cycle control. Dysregulation of UBE2A has been linked to neurological disorders, such as X-linked intellectual disability (XLID), and certain cancers, highlighting its importance in maintaining genomic stability and cellular homeostasis.
UBE2A antibodies are widely used in research to detect and quantify UBE2A protein expression, assess post-translational modifications, and investigate its interactions with substrates like RAD18 in DNA repair pathways. These antibodies are essential in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. Specificity and validation across species (e.g., human, mouse) are critical for reliable experimental outcomes. Recent studies also explore UBE2A's role in neurodegenerative diseases and cancer progression, making its antibody a valuable resource for uncovering molecular mechanisms and potential therapeutic targets. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and compatibility with diverse experimental models to ensure robust reproducibility.
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