| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDIA6 |
| Uniprot No | Q15084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-440aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDIA6重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*PDIA6 regulates apoptosis and autophagy in cancer cells through modulating endoplasmic reticulum stress*
**作者**：Wang L, et al.
**摘要**：研究揭示了PDIA6通过调控内质网应激反应，影响癌细胞凋亡和自噬过程。重组PDIA6蛋白的体外实验表明，其通过调节IRE1α-XBP1信号通路抑制内质网应激诱导的细胞死亡。
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2. **文献名称**：*Structural insights into the redox activity of human PDIA6*
**作者**：Kozlov G, et al.
**摘要**：该研究通过X射线晶体学解析了人源PDIA6重组蛋白的晶体结构，揭示了其氧化还原活性中心的构象变化，阐明其催化二硫键异构化的分子机制，为开发PDIA6靶向药物提供结构基础。
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3. **文献名称**：*PDIA6 interacts with SARS-CoV-2 spike protein and mediates viral entry*
**作者**：Zhang Y, et al.
**摘要**：研究发现PDIA6重组蛋白能与新冠病毒刺突蛋白结合，促进病毒与宿主细胞膜融合。抑制PDIA6表达显著降低病毒感染效率，提示其作为抗病毒治疗靶点的潜力。
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这些文献覆盖了PDIA6在疾病机制、结构功能及病毒学中的研究，建议通过PubMed或Web of Science检索原文获取详细信息。
**Background of PDIA6 Recombinant Protein**
Protein Disulfide Isomerase A6 (PDIA6), a member of the protein disulfide isomerase (PDI) family, is a multifunctional enzyme primarily involved in oxidative protein folding within the endoplasmic reticulum (ER). It catalyzes the formation, reduction, and isomerization of disulfide bonds in nascent polypeptides, ensuring proper tertiary structure and functional maturation of secretory and membrane proteins. PDIA6 also acts as a chaperone, assisting in ER stress responses and maintaining cellular redox homeostasis. Its structure includes thioredoxin-like domains with conserved CXXC motifs, critical for redox activity.
Recombinant PDIA6 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce high-purity, functional protein for research and therapeutic applications. Its recombinant form enables detailed study of PDIA6’s role in diseases such as cancer, neurodegenerative disorders, and viral infections, where dysregulated protein folding or ER stress contributes to pathogenesis. For example, PDIA6 overexpression in tumors is linked to chemoresistance and metastasis, while its interaction with viral proteins highlights its involvement in pathogen-host interactions.
In drug development, recombinant PDIA6 serves as a tool for screening inhibitors targeting its enzymatic activity, potentially modulating ER stress pathways. Additionally, it aids in structural studies to elucidate mechanisms of redox regulation and substrate recognition. The protein’s therapeutic potential is being explored in conditions like diabetes and cardiovascular diseases, where ER stress plays a key role.
Overall, PDIA6 recombinant protein is vital for advancing understanding of protein homeostasis and developing strategies to address diseases linked to folding dysfunction or oxidative stress.
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