| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L48MT; CGI-118; HSPC290; MRP-L48 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL48 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL48抗体的3篇参考文献的简要信息：
1. **文献名称**: "MRPL48 is essential for mitochondrial ribosome assembly and function in human cells"
**作者**: Smith J, et al.
**摘要**: 研究利用CRISPR敲除和MRPL48特异性抗体，发现MRPL48缺失导致线粒体核糖体组装缺陷，呼吸链活性下降，提示其在维持线粒体翻译中的关键作用。
2. **文献名称**: "Characterization of mitochondrial ribosomal protein L48 and its role in oxidative phosphorylation"
**作者**: Chen L, et al.
**摘要**: 通过免疫印迹(使用兔多克隆MRPL48抗体)和免疫电镜技术，证实MRPL48定位于线粒体大亚基，其表达水平与细胞能量代谢异常相关。
3. **文献名称**: "Dysregulation of MRPL48 expression in cancer cells correlates with mitochondrial dysfunction"
**作者**: Wang Y, et al.
**摘要**: 该研究利用抗MRPL48抗体分析多种癌细胞系，发现其表达下调与线粒体膜电位丧失和凋亡增加相关，提示其作为肿瘤治疗潜在靶点。
注：以上文献为示例，实际引用时需查询具体数据库(如PubMed)获取真实文献。
The MRPL48 antibody is a research tool designed to detect the mitochondrial ribosomal protein L48. a component of the mitochondrial large ribosomal subunit. MRPL48 is part of the mitochondrial ribosome machinery essential for synthesizing proteins encoded by mitochondrial DNA (mtDNA), which are critical for oxidative phosphorylation (OXPHOS) and cellular energy production. This protein plays a role in ribosome assembly, stability, and mitochondrial translation, impacting metabolic homeostasis and mitochondrial function.
Antibodies targeting MRPL48 are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression, localization, and interactions in various tissues or cell lines. Researchers employ these antibodies to investigate mitochondrial disorders, metabolic diseases, and cancers linked to mitochondrial dysfunction, as altered MRPL48 expression may correlate with impaired energy metabolism or disease progression.
Studies utilizing MRPL48 antibodies have contributed to understanding mitochondrial ribosome biogenesis, dynamics, and their implications in aging, neurodegeneration (e.g., Parkinson’s or Alzheimer’s disease), and cancer metabolism. Validated antibodies are crucial for specificity, as cross-reactivity with cytoplasmic ribosomal proteins can occur. Overall, MRPL48 antibodies serve as vital reagents for exploring mitochondrial biology and its connections to human health.
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