首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体C1q亚基A(C1QA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QA含量。C1QA 是补体经典途径起始分子 C1 的亚基之一。它在免疫调节、清除凋亡细胞等过程有重要作用。研究发现其参与炎症反应、神经退行性疾病等病理机制，还与肿瘤免疫逃逸相关，对其深入研究有望为相关疾病治疗提供新靶点。试剂盒检测范围为0.156 μg/mL-10 μg/mL，适用于探究小鼠模型中补体系统的激活机制、免疫相关疾病病理过程以及药物干预对C1QA表达的影响等基础研究，为免疫学、分子病理学等领域提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1qaComplement C1q subcomponent subunit A ELISA Kit
缩写：C1QA
Uniprot No.：P98086
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C1QA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 95 | |||||
| Range % | 90-110 | ||||||
| 1:10 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 87-101 | ||||||
| 1:40 | Average % | 96 | |||||
| Range % | 85-105 | ||||||
回收率：
| The recovery of mouse C1QA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 88-99 | |||||
| EDTA plasma (n=4) | 93 | 86-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μg/mlOD1OD2AverageCorrected102.5982.5512.5752.48851.4521.4841.4681.3812.50.7930.8050.7990.7121.250.4340.4220.4280.3410.6250.2690.2570.2630.1760.3120.1760.1820.1790.0920.1560.1220.1190.1210.03400.0890.0850.087 | |||||||
货期：3-5 working days
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