| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | I-MF; I-mfa |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human MDFI |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MDFI(MyoD Family Inhibitor)抗体的3篇虚构参考文献示例(注意：以下文献为示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Characterization of MDFI Antibody in Myogenic Differentiation"*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性MDFI抗体探究其在肌肉分化中的作用，通过Western blot和免疫荧光证实MDFI蛋白在成肌细胞中的表达动态变化，揭示其对MyoD家族转录因子的抑制作用。
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2. **文献名称**: *"MDFI as a Tumor Suppressor: Insights from Immunohistochemical Analysis"*
**作者**: Zhang L, et al.
**摘要**: 通过开发高亲和力MDFI单克隆抗体，分析多种癌症组织中MDFI的表达水平，发现其低表达与肿瘤侵袭性相关，提示MDFI可能作为抑癌基因参与调控细胞周期。
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3. **文献名称**: *"Development and Validation of a Polyclonal Antibody for MDFI Detection"*
**作者**: Lee J, et al.
**摘要**: 报道一种兔源多克隆MDFI抗体的制备方法，验证其在ELISA、免疫沉淀及组织切片中的特异性，为研究MDFI在胚胎发育中的功能提供工具。
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**注意**：以上文献为示例，实际研究中请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索真实文献(关键词：MDFI antibody, I-mfa, MyoD inhibitor)。真实文献可能涉及MDFI在细胞分化、癌症或神经发育中的具体机制。
**Background of MDFI Antibody**
MDFI (MyoD family inhibitor), also known as I-mfa (Inhibitor of MyoD family a), is a cytoplasmic protein that regulates cellular differentiation and developmental processes by modulating transcription factors. It interacts with and inhibits the activity of basic helix-loop-helix (bHLH) transcription factors, such as MyoD and myogenin, which are critical for muscle development. Additionally, MDFI binds to β-catenin, a key effector of the Wnt signaling pathway, influencing cell proliferation and differentiation.
MDFI contains two conserved domains: the N-terminal I-mfa domain, responsible for protein-protein interactions, and the C-terminal CAAX motif, which facilitates membrane association. Its dual role in suppressing myogenic differentiation and modulating Wnt signaling makes it a focus in studies of muscle biology, neurodevelopment, and cancer.
Antibodies targeting MDFI are essential tools for investigating its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to study MDFI's regulatory mechanisms in vitro and in vivo. Validated MDFI antibodies help researchers explore its involvement in diseases, such as muscular dystrophies and malignancies, and its crosstalk with signaling pathways. Specificity and sensitivity are critical for these antibodies, often confirmed using knockout cell lines or tissues to ensure reliable experimental outcomes.
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