| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDZD11 |
| Uniprot No | Q5EBL8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDSRIPYDDYPVVFLPAYENPPAWIPP HERVHHPDYNNELTQFLPRTITLKKPPGAQLGFNIRGGKASQLGIFISKV IPDSDAHRAGLQEGDQVLAVNDVDFQDIEHSKAVEILKTAREISMRVRFF PYNYHRQKERTVH |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDZD11重组蛋白的参考文献示例，涵盖不同研究方向：
1. **标题**: Structural Characterization of Recombinant PDZD11 and Its Interaction with PDZ Domains
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统纯化了重组PDZD11蛋白，通过X射线晶体学解析其三维结构，揭示了其PDZ结构域与SAP97蛋白C末端肽段的特异性结合，为突触后蛋白质网络的调控机制提供新见解。
2. **标题**: PDZD11 Regulates Cell Migration by Modulating β-Catenin Signaling
**作者**: Zhang L, et al.
**摘要**: 该研究发现重组PDZD11通过直接结合β-catenin增强Wnt信号通路活性，促进肿瘤细胞迁移和侵袭。临床数据分析显示，PDZD11高表达与结直肠癌患者预后不良相关。
3. **标题**: Recombinant PDZD11 Facilitates the Study of ALG-2 Dependent Apoptosis Mechanisms
**作者**: Tanaka K, et al.
**摘要**: 利用昆虫细胞表达系统制备的高纯度PDZD11重组蛋白，揭示了其与ALG-2的相互作用在内质网应激诱导的细胞凋亡中的关键作用。阻断该相互作用可显著减少细胞死亡，为靶向治疗提供新策略。
4. **标题**: Functional Analysis of PDZD11 in Neuronal Development Using Recombinant Protein
**作者**: Müller R, et al.
**摘要**: 通过体外神经元模型，证实重组PDZD11蛋白通过结合神经连接蛋白促进神经突生长和分支，提示其在神经发育和损伤修复中的潜在应用价值。
*注：以上文献为示例性质，作者及内容为虚构，仅供参考。实际研究中建议通过学术数据库检索真实文献。*
PDZD11 (PDZ domain-containing protein 11), also known as SAPIS or PAPIN, is a multi-functional scaffold protein involved in cellular signaling, adhesion, and trafficking processes. It belongs to the PDZ domain protein family, characterized by conserved PDZ domains that mediate protein-protein interactions by binding to C-terminal motifs or internal sequences of partner proteins. PDZD11 contains two PDZ domains, enabling it to act as an adaptor molecule that organizes molecular complexes at specific subcellular locations.
Biologically, PDZD11 plays roles in regulating cell migration, endocytic trafficking, and epithelial polarization. It interacts with diverse proteins, including the tyrosine phosphatase PTP-BL, the Rab5-GEF RIN3. and junctional proteins like nectin-1. linking signaling pathways to cytoskeletal dynamics. Studies suggest its involvement in cancer progression, as altered PDZD11 expression correlates with metastasis in certain malignancies. Additionally, it contributes to neuronal development by modulating synaptic protein clustering.
Recombinant PDZD11 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and functional mechanisms. Produced via bacterial or mammalian expression systems, the recombinant form often includes tags (e.g., GST, His-tag) for purification and detection. Researchers utilize it in pull-down assays, structural analyses (e.g., X-ray crystallography), and screening for small-molecule inhibitors targeting PDZ domain-mediated interactions. Its applications extend to disease modeling, particularly in cancers and neurological disorders where PDZD11 dysregulation is implicated. The recombinant protein serves as a critical tool for elucidating its pathophysiological roles and therapeutic potential.
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