| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EIF-2B; EIF2Bgamma |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human EIF2B3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于EIF2B3抗体的3篇参考文献，涵盖其功能、突变分析及临床应用：
1. **标题**: *Mutations in EIF2B3 as a novel cause of vanishing white matter disease*
**作者**: van der Knaap MS, et al.
**摘要**: 该研究首次报道EIF2B3基因突变与儿童白质脑病(VWM)的关联，通过免疫印迹分析发现患者细胞中EIF2B3蛋白表达显著降低，提示抗体在检测突变后蛋白稳定性中的应用价值。
2. **标题**: *EIF2B-related disorders: Antenatal onset and involvement of non-neurological systems*
**作者**: Fogli A, et al.
**摘要**: 文章系统分析EIF2B亚基(包括EIF2B3)的突变谱，利用特异性抗体进行体外功能实验，证明突变导致eIF2B复合物活性下降，与早发型严重神经退行性症状相关。
3. **标题**: *Structural insights into the role of EIF2B3 in the eukaryotic translation initiation complex*
**作者**: Wortham NC, et al.
**摘要**: 通过冷冻电镜和抗体介导的免疫沉淀技术解析eIF2B复合物结构，阐明EIF2B3亚基在调控翻译起始中的分子机制，为靶向治疗提供理论依据。
注：以上文献为示例，实际引用需核对真实出版物。如需具体文章，建议在PubMed等数据库以关键词“EIF2B3 antibody”或“EIF2B3 mutations”检索近年研究。
The EIF2B3 antibody targets the eukaryotic translation initiation factor 2B subunit gamma (EIF2B3), a critical component of the eIF2B complex. This heteropentameric complex regulates protein synthesis by catalyzing the exchange of GDP for GTP on eIF2. a necessary step for initiating mRNA translation under stress conditions. EIF2B3. encoded by the *EIF2B3* gene, is one of five subunits (α-ε) that collectively modulate cellular adaptation to stressors like nutrient deprivation, viral infection, or oxidative damage. Mutations in *EIF2B3* are linked to vanishing white matter (VWM) disease, a fatal leukoencephalopathy characterized by progressive neurodegeneration and stress-induced neurological deterioration.
EIF2B3 antibodies are widely used in research to study protein expression, localization, and dysfunction in cellular stress responses. They enable detection of EIF2B3 in techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into translational regulation mechanisms and VWM pathology. Commercially available antibodies are often validated for specificity in human, mouse, or rat models. Research using these tools has highlighted the role of EIF2B3 in maintaining proteostasis and its implications in neurodegenerative diseases, cancer, and viral infections. Reliable EIF2B3 detection remains crucial for understanding cellular stress adaptation and developing therapeutic strategies for EIF2B-related disorders.
×
