首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体片断3a(C3a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的C3a含量。C3a是重要的补体激活产物。在生理情况下参与免疫调节、组织修复等。其研究机制主要聚焦于免疫反应调控，它能通过与受体结合，影响免疫细胞功能，在炎症、自身免疫病等病理过程中发挥关键作用，是潜在治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础免疫学研究中补体系统激活机制的探索、炎症性疾病动物模型的生物标志物分析，以及药物研发中抗炎或免疫调节剂的效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C3a
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:80 | Average % | 88 | ||||
| Range % | 82-95 | |||||
回收率：
| The recovery of mouse C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-104 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6382.6932.6662.49552.2242.1322.1782.0072.51.4031.3881.3961.2251.250.8630.8290.8460.6750.6250.5870.5660.5770.4060.3120.4530.4320.4430.2720.1560.2990.2750.2870.11600.1640.1780.171 | ||||||
货期：3-5 working days
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