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产品描述：鱼补体蛋白3(C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的C3含量。C3是补体系统重要成分。其背景在于参与免疫防御、免疫自稳等。研究机制方面，它可被多种途径激活，裂解为多个片段，参与调理吞噬、介导炎症反应等，在抗感染、自身免疫病等研究中有重要价值。试剂盒检测范围为12.5 μg/mL-500 μg/mL，该产品广泛应用于水产动物免疫机制研究，如细菌或病毒感染模型中的补体通路分析、养殖环境压力对免疫功能的评估，以及疫苗或免疫增强剂的功效验证等实验场景，为水生生物健康管理和疾病防控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C3
种属：Fish
样本类型：serum, plasma
检测范围：12.5 μg/mL-500 μg/mL
灵敏度：12.5 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-108 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 5000.1760.1740.175 2500.3810.3930.387 1150.5570.5690.563 37.50.8940.9010.898 12.51.4811.4981.490 02.4172.5422.480 | ||||||
货期：3-5 working days
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