首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体蛋白3(C3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3含量。C3即补体C3，属于补体系统核心成分。其背景在于它参与固有免疫和适应性免疫应答。研究机制方面，C3可被多种途径激活，裂解产物参与调理吞噬、免疫黏附、介导炎症反应等，在抗感染、免疫病理等过程有重要作用。试剂盒检测范围为93.75 ng/mL-6000 ng/mL，适用于小鼠疾病模型（如脓毒症、关节炎或肾损伤模型）中补体系统激活状态的评估，或用于免疫调节药物开发中C3动态变化的监测，为免疫相关机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C3Complement C3 ELISA kit; HSE-MSF) [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
缩写：C3
Uniprot No.：P01027
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 ng/mL-6000 ng/mL
灵敏度：23.44 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 100 | ||||
| Range % | 94-106 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 83-96 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:8000 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of mouse C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 60002.2032.0362.1202.034 30001.3651.2761.3211.235 15000.7640.7040.7340.648 7500.4250.3970.4110.325 3750.2870.2940.2910.205 187.50.2120.1960.2040.118 93.750.1560.1590.1580.072 00.0870.0840.086 | ||||||
货期：3-5 working days
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