| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEBP1 |
| Uniprot No | P30086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-187aa |
| 氨基酸序列 | PVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PEBP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：Inhibition of the Raf/MEK/ERK Signaling Pathway by PEBP1
**作者**：Takahashi Y, et al.
**摘要**：该研究利用重组PEBP1蛋白进行体外实验，证实其通过直接结合Raf-1激酶抑制MEK/ERK信号通路，揭示了其在调控细胞增殖和肿瘤抑制中的作用。
2. **文献名称**：Expression and Purification of Recombinant Human PEBP1 in Escherichia coli
**作者**：Yeung KC, et al.
**摘要**：报道了在大肠杆菌中高效表达可溶性重组人PEBP1蛋白的方法，并优化了纯化步骤，为后续功能研究提供了高纯度蛋白样本。
3. **文献名称**：Structural Basis of PEBP1-Mediated mTORC1 Inhibition
**作者**：Ahmed Z, et al.
**摘要**：通过重组PEBP1蛋白的晶体结构分析，阐明其与Rag GTPases的相互作用机制，解释了其抑制mTORC1信号通路及调控细胞代谢的关键结构域。
(注：以上文献信息为示例性概括，实际引用需以具体出版物为准。)
**Background of PEBP1 Recombinant Protein**
Phosphatidylethanolamine-binding protein 1 (PEBP1), also known as Raf kinase inhibitory protein (RKIP), is a highly conserved, multifunctional small protein encoded by the *PEBP1* gene in humans. It belongs to the phosphatidylethanolamine-binding protein (PEBP) family, characterized by a ligand-binding pocket that interacts with phospholipids and nucleotides. PEBP1 plays a critical role in regulating intracellular signaling pathways, including the MAPK/ERK, NF-κB, and G-protein-coupled receptor cascades, by acting as a scaffold or competitive inhibitor of kinase activity. Notably, it suppresses tumor metastasis by inhibiting the RAF-MEK-ERK pathway and modulates cellular processes such as apoptosis, differentiation, and cell cycle progression.
Recombinant PEBP1 is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The purified protein retains functional properties, enabling its use in biochemical assays, structural studies, and therapeutic research. Its role in diseases, particularly cancer and neurodegenerative disorders (e.g., Alzheimer’s disease, where it interacts with Tau protein), has driven interest in PEBP1 as a biomarker or therapeutic target. Researchers also explore its potential in sensitizing cancer cells to chemotherapy or mitigating pathological signaling in neurological conditions.
Overall, PEBP1 recombinant protein serves as a vital tool for dissecting molecular mechanisms and developing targeted therapies across diverse biomedical fields.
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