首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠降钙素(CT)酶联免疫试剂盒检测血清、血浆、组织培养上清液、组织匀浆样本中的CALCA含量。CALCA 是重要靶点，它编码降钙素基因相关肽等，在痛觉传导、血管调节等生理过程起关键作用。研究机制上，聚焦其信号通路及受体相互作用，旨在通过药物干预该靶点，为偏头痛、心血管病等疾病治疗提供新思路。试剂盒检测范围为12.5 pg/mL-800 pg/mL，本产品专为科研实验设计，可广泛应用于小鼠疾病模型构建、细胞因子调控机制研究、药物干预效果评估等基础研究领域，为代谢性疾病及骨骼系统研究提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CALCA Calcitonin
缩写：CALCA
Uniprot No.：P70160
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 84 | |
| Range % | 80-88 | ||
| 1:2 | Average % | 95 | |
| Range % | 91-99 | ||
| 1:4 | Average % | 86 | |
| Range % | 83-89 | ||
| 1:8 | Average % | 97 | |
| Range % | 92-102 |
回收率：
| The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-93 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 8002.0262.0682.0471.945 4001.4121.4651.4391.337 2000.9080.9350.9220.820 1000.5680.5340.5510.449 500.3460.3260.3360.234 250.2690.2680.2690.167 12.50.1890.1980.1940.092 00.1010.1030.102 | ||||||
货期：3-5 working days
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