首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠降钙素(CT)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的CALCA含量。CALCA 是重要靶点。它在人体生理过程有诸多参与。对其研究机制主要从基因表达调控等方面探究，目的是明确它在疾病发生发展中的作用，为开发针对该靶点的药物、开展精准治疗等奠定基础，有望推动相关疾病治疗的进步。试剂盒检测范围为1.56 pg/mL-100 pg/mL，大鼠模型中的钙代谢调控机制探索、骨骼疾病动物模型评估、甲状腺相关肿瘤分子机制研究等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CALCA Calcitonin
缩写：CALCA
Uniprot No.：P01257
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 94 | |
| Range % | 87-101 | ||
| 1:2 | Average % | 88 | |
| Range % | 80-95 | ||
| 1:4 | Average % | 95 | |
| Range % | 86-106 | ||
| 1:8 | Average % | 90 | |
| Range % | 82-100 |
回收率：
| The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-99 | ||||
| EDTA plasma (n=4) | 94 | 88-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.7462.7792.7632.582 502.1382.1752.1571.976 251.3951.4531.4241.243 12.50.8660.8540.8600.679 6.250.5520.5620.5570.376 3.120.4280.4010.4150.234 1.560.2830.2980.2910.110 00.1790.1830.181 | ||||||
货期：3-5 working days
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