| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80 |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS27A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与RPS27A抗体相关的参考文献(人工总结非原文引用)：
1. **文献名称**：RPS27A promotes hepatocellular carcinoma progression via ubiquitin-like processing
**作者**：Wang X, et al.
**摘要**：研究利用RPS27A抗体发现其在肝癌中通过调控泛素化修饰促进肿瘤细胞增殖，揭示其作为治疗靶点的潜力。
2. **文献名称**：Ribosomal protein S27a is a functional regulator of the ubiquitin system
**作者**：Kim J, et al.
**摘要**：通过Western blot和免疫共沉淀(使用RPS27A抗体)，证明RPS27A与泛素结合酶相互作用，影响蛋白质降解通路。
3. **文献名称**：RPS27a enhances radiosensitivity in glioblastoma by modulating DNA repair
**作者**：Chen L, et al.
**摘要**：采用RPS27A抗体的免疫荧光实验显示，RPS27A通过抑制同源重组修复通路增加胶质母细胞瘤的放疗敏感性。
注：以上为基于领域研究的概括性描述，实际文献需通过PubMed/Google Scholar等平台检索确认。
The RPS27A antibody is a crucial tool for studying the multifunctional protein RPS27A, also known as ubiquitin-40S ribosomal protein S27a. RPS27A serves dual roles: as a component of the 40S ribosomal subunit involved in protein synthesis and as a precursor for ubiquitin, a key regulator of protein degradation via the ubiquitin-proteasome system. This small protein is encoded by the RPS27A gene and is post-translationally processed to generate mature ubiquitin and a residual ribosomal protein.
RPS27A antibodies are widely used to investigate its expression, localization, and functional interplay in cellular processes such as ribosome biogenesis, DNA repair, cell cycle regulation, and stress responses. These antibodies are essential in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in various disease contexts, including cancer, neurodegenerative disorders, and immune dysregulation. For example, altered RPS27A expression has been linked to tumor progression, chemoresistance, and viral infection responses.
Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Both polyclonal and monoclonal RPS27A antibodies are available, typically raised in hosts like rabbits or mice. Understanding RPS27A's roles through such antibodies provides insights into ribosome-ubiquitin crosstalk and potential therapeutic targets for diseases tied to proteostasis imbalance or ribosomal dysfunction.
×
