| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MNK2; GPRK7 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MKNK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及MKNK2抗体的参考文献及其关键信息：
1. **文献名称**：The role of MNK2 in hepatocellular carcinoma development
**作者**：Li Y, et al.
**摘要**：研究使用兔源MNK2多克隆抗体(货号ab12345)检测肝癌组织中的MNK2蛋白表达水平，发现MNK2通过调控eIF4E磷酸化促进肿瘤血管生成。
2. **文献名称**：Phospho-MKNK2 (Thr465) antibody validation in glioblastoma models
**作者**：Zhang H, et al.
**摘要**：开发了一种特异性识别MNK2 Thr465磷酸化位点的小鼠单克隆抗体(克隆号4D7)，通过免疫沉淀和质谱验证其在胶质母细胞瘤细胞系中的靶标特异性。
3. **文献名称**：MAPK-interacting kinases as therapeutic targets in breast cancer
**作者**：Gupta S, et al.
**摘要**：比较了市售MNK1/MNK2抗体(包括CST#3481和Santa Cruz sc-28700)在乳腺癌组织微阵列中的交叉反应性，发现sc-28700对MNK2亚型具有更高选择性。
注：以上为模拟数据，实际文献需通过PubMed/Google Scholar检索关键词如"MNK2 antibody validation"或结合抗体货号查询。如需真实文献，建议补充具体抗体生产商或应用场景进一步筛选。
The MKNK2 antibody is a research tool designed to detect and analyze the mitogen-activated protein kinase (MAPK)-interacting serine/threonine kinase 2 (MKNK2), a protein involved in cellular signaling pathways. MKNK2. also known as MNK2. is part of the MAPK cascade and regulates mRNA translation by phosphorylating eukaryotic initiation factor 4E (eIF4E), influencing protein synthesis under stress or growth stimuli. It exists in two isoforms (MNK2a and MNK2b) due to alternative splicing, with distinct roles in cell proliferation, survival, and stress responses.
MKNK2 antibodies are widely used in Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and activity in various tissues and cell lines. Researchers employ these antibodies to explore MKNK2’s interaction with upstream regulators (e.g., ERK, p38 MAPK) and downstream targets, particularly in cancer biology and inflammatory diseases. Notably, MKNK2’s dual role as a tumor promoter or suppressor, depending on cellular context, has spurred interest in its therapeutic targeting.
Studies using MKNK2 antibodies have linked its dysregulation to cancers, neurodegenerative disorders, and immune dysfunctions. Validated antibodies help clarify its contribution to drug resistance, metastasis, and survival pathways. As MKNK2 inhibitors enter clinical trials, these antibodies remain critical for mechanistic insights and biomarker development, underscoring their importance in translational research.
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