| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DERM4; TMEM9A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TMEM9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TMEM9抗体的3篇参考文献示例(内容为模拟，仅供参考)：
1. **标题**: *TMEM9 regulates lysosomal exocytosis and promotes cancer metastasis*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫荧光和Western blot技术，利用TMEM9抗体揭示了TMEM9在调控溶酶体胞吐中的作用，并发现其高表达与癌症转移相关。
2. **标题**: *TMEM9 modulates Wnt/β-catenin signaling in colorectal cancer*
**作者**: Lee S, Kim DH.
**摘要**: 作者使用TMEM9抗体进行免疫组化分析，发现TMEM9通过增强Wnt信号通路促进结直肠癌细胞增殖，提示其作为潜在治疗靶点。
3. **标题**: *Characterization of TMEM9 as a novel biomarker in gastric inflammation*
**作者**: Wang X, et al.
**摘要**: 研究利用TMEM9抗体在小鼠模型中检测蛋白表达，表明TMEM9在胃黏膜炎症反应中异常表达，可能与免疫调节相关。
**备注**：若需真实文献，建议通过PubMed或Google Scholar搜索关键词“TMEM9 antibody”或“TMEM9 immunohistochemistry”，并筛选实验方法部分明确提及该抗体的研究。
The TMEM9 antibody is a research tool used to study the transmembrane protein 9 (TMEM9), a conserved lysosomal and endosomal membrane-associated protein implicated in vesicle trafficking, lysosomal acidification, and V-ATPase assembly. TMEM9 is characterized by a single transmembrane domain and interacts with vacuolar ATPase (V-ATPase), a key regulator of intracellular pH. Its role in modulating V-ATPase activity links TMEM9 to cellular processes like autophagy, nutrient sensing, and tumor progression. Dysregulation of TMEM9 has been observed in cancers, including colorectal, hepatocellular, and breast cancers, where it promotes proliferation, metastasis, and chemoresistance.
TMEM9 antibodies are primarily used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect TMEM9 expression and localization in tissues or cell lines. They help validate TMEM9's overexpression in cancer models and its association with poor prognosis. Additionally, these antibodies aid in elucidating TMEM9's molecular mechanisms, such as its interaction with V-ATPase subunits or involvement in Wnt/β-catenin and mTOR signaling pathways. Commercial TMEM9 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and require validation for species reactivity (e.g., human, mouse) and application-specific compatibility. Research using TMEM9 antibodies continues to explore its potential as a therapeutic target or biomarker in oncology and lysosome-related disorders.
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