| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CRCS1; GalNAc-T12 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human GALNT12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GALNT12抗体的3篇代表性文献的简要信息：
1. **文献名称**：*"Mutations in GALNT12 in individuals with colorectal cancer"*
**作者**：Gu C. et al. (2008)
**摘要**：该研究通过基因测序发现结直肠癌患者中GALNT12基因的胚系突变，并利用特异性抗体检测其蛋白表达水平，表明突变导致GALNT12酶活性丧失，可能通过影响粘蛋白O-糖基化促进肿瘤发生。
2. **文献名称**：*"GALNT12 expression predicts prognosis in gastric cancer"*
**作者**：Li Y. et al. (2015)
**摘要**：通过免疫组化分析GALNT12在胃癌组织中的表达，发现其低表达与患者不良预后相关，抗体实验证实其通过调控EGFR信号通路抑制肿瘤侵袭转移。
3. **文献名称**：*"Functional characterization of GALNT12 in pancreatic cancer progression"*
**作者**：Wang Q. et al. (2020)
**摘要**：利用GALNT12特异性抗体进行Western blot和免疫荧光实验，揭示其在胰腺癌中通过修饰MUC1蛋白的糖基化状态影响肿瘤细胞粘附及转移能力，提示其作为潜在治疗靶点。
注：以上文献为示例性概括，具体引用时需核对真实文献来源及细节。
GALNT12 (polypeptide N-acetylgalactosaminyltransferase 12) is a member of the glycosyltransferase family that initiates mucin-type O-glycosylation by transferring N-acetylgalactosamine (GalNAc) to serine or threonine residues on target proteins. This post-translational modification plays critical roles in protein stability, trafficking, and cell-cell interactions. GALNT12 has garnered attention due to its association with colorectal cancer (CRC). Germline mutations in GALNT12 were identified in familial CRC cases, suggesting its potential role as a tumor suppressor. Dysregulated GALNT12 expression may contribute to tumor progression by altering O-glycosylation patterns of oncoproteins or adhesion molecules, thereby influencing cell signaling, metastasis, and immune evasion.
GALNT12 antibodies are essential tools for detecting GALNT12 protein expression, localization, and functional studies. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to assess GALNT12 levels in tissues or cell lines. Research applications include investigating GALNT12's involvement in cancer biology, its interaction with substrates (e.g., MUC1. EGFR), and its regulatory mechanisms. Commercially available GALNT12 antibodies are typically raised against specific epitopes, such as peptide sequences from the N-terminal or catalytic domains. Validation data (e.g., knockout controls) are crucial to ensure specificity, given homology among GALNT family members. Studying GALNT12 may offer insights into CRC pathogenesis and therapeutic targeting of O-glycosylation pathways.
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