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产品描述：鱼过氧化氢酶(CAT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CAT含量。试剂盒检测范围为125 mU/mL-2000 mU/mL，该产品可应用于水产养殖研究中鱼类健康状态监测、水体污染对生物抗氧化系统的影响评估，以及环境毒理学中重金属、有机污染物等胁迫因子的效应机制研究，为鱼类生理生化研究提供可靠的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CAT
Uniprot No.：H2L8K1
种属：Fish
样本类型：serum, plasma
检测范围：125 mU/mL-2000 mU/mL
灵敏度：62.5 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-105 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 88 | 83-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2Average 20000.0910.0890.090 10000.2420.2570.250 5000.5530.5870.570 2500.9130.9800.947 1251.6471.5631.605 02.3812.3192.350 | ||||||
货期：3-5 working days
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