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产品描述：鱼胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCK含量。CCK（胆囊收缩素）是一种胃肠道及神经系统分泌的激素，主要作用是刺激胆囊收缩和促进胰腺分泌。其研究机制涉及通过靶细胞表面的受体发挥作用，调节胃肠运动、食欲调节、激素分泌等生理过程。CCK在消化系统和神经系统中的作用密切相关，通过脑肠轴实现双向调节，影响多种生理和病理过程。试剂盒检测范围为200 pg/mL-5000 pg/mL，本试剂盒适用于实验室环境下的样本检测，可应用于水产养殖研究、鱼类生理学实验或环境毒理学评估等场景，为水生生物基础研究提供高灵敏度的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CCK
Uniprot No.：D7S9W7
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：200 pg/mL-5000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-105 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-105 | |||||
回收率：
| The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-91 | ||||
| EDTA plasma (n=4) | 95 | 86-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average50000.1370.1350.13620000.2410.2280.23512000.3240.3390.3325000.6160.5770.5972001.2351.2741.25502.6022.6962.649 | ||||||
货期：3-5 working days
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