首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠克拉拉细胞蛋白(CC16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CC16含量。CC16是一种由呼吸道上皮的club细胞分泌的蛋白质，具有抗炎、免疫调节、抗纤维化等多种生物学功能。研究表明，CC16在慢性气道疾病的发病机制中起到重要作用，可能成为慢性气道疾病的潜在生物标志物和治疗靶点。CC16通过抑制炎症、调节细胞因子表达和阻断NF-κB信号通路等机制发挥其保护作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于小鼠呼吸系统疾病模型研究，包括肺损伤评估、炎症反应监测及环境污染物暴露研究，尤其适合通过血清或血浆样本动态追踪体内CC16水平变化，或通过肺组织匀浆分析局部蛋白表达。为科研人员探究CC16在肺部病理生理机制中的作用提供可靠工具，也可用于药物干预效果评价中的生物标志物检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Scgb1a1 ELISA Kit; Cc10 ELISA Kit; Ugb ELISA Kit; Utg ELISA Kit; Uteroglobin ELISA Kit; Clara cell 17 kDa protein ELISA Kit; Clara cell phospholipid-binding protein ELISA Kit; CCPBP ELISA Kit; Clara cells 10 kDa secretory protein ELISA Kit; CC10 ELISA Kit; PCB-binding protein ELISA Kit; Secretoglobin family 1A member 1 ELISA Kit
缩写：CC16
Uniprot No.：Q06318
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 91-103 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of mouse CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0951.9622.0291.86851.2681.1761.2221.0612.50.7550.7040.7300.5691.250.4900.4690.4800.3190.6250.3360.3240.3300.1690.3120.2650.2590.2620.1010.1560.2120.2090.2110.05000.1640.1570.161 | ||||||
货期：3-5 working days
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