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产品描述：猪单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CCL2含量。CCL2是重要的趋化因子。它能招募单核细胞、记忆性T淋巴细胞等至炎症部位，在炎症反应、免疫调节等过程中发挥关键作用。研究发现其与肿瘤发生发展、神经退行性疾病等相关，围绕其机制的研究旨在为相关疾病治疗提供新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于动物疾病模型研究、免疫调控机制探索及炎症相关分子通路分析，例如猪病毒感染、细菌性肺炎等病理过程中单核细胞趋化活性的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：CCL2
Uniprot No.：P42831
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of pig MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-96 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0782.0692.0741.88010001.6101.6871.6491.4555001.0991.0591.0790.8852500.6150.6380.6270.4331250.4270.4380.4330.23962.50.3420.3490.3460.15231.250.2760.2680.2720.07800.1890.1980.194 | ||||||
货期：3-5 working days
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