首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：豚鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL2含量。CCL2是一种趋化因子，在免疫调节、炎症反应等生理病理过程中扮演重要角色。研究发现它能募集单核细胞等免疫细胞到炎症部位。其异常表达与肿瘤进展、心血管疾病等密切相关，探索CCL2作用机制有助于疾病治疗新策略的开发。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，可应用于炎症机制研究、免疫调节实验、疾病模型建立等基础研究领域。例如在豚鼠感染模型或自身免疫性疾病模型中，可通过监测血清或组织样本中MCP - 1的动态变化，揭示疾病进程中趋化因子的调控规律。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCL2 ELISA Kit; MCP1 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：CCL2
Uniprot No.：Q08782
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of guinea pig MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15001.8571.9021.8801.6807501.3451.4031.3741.1743750.9810.9540.9680.7681870.7480.7000.7240.524940.5110.5320.5220.322470.4160.4260.4210.22123.50.2890.2810.2850.08500.2010.1990.200 | ||||||
货期：3-5 working days
×
