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Mouse macrophage inflammatory protein 3β,MIP-3β ELISA kit

  • 中文名： 小鼠巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA Kit
货号: IPD-E04671m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL19含量。CCL19，即趋化因子CCL19，是一种小分子蛋白，主要在树突状细胞、T细胞和多种肿瘤细胞上表达。它通过与其受体CCR7结合，在免疫系统中发挥重要作用，如趋化树突状细胞和T细胞浸润肿瘤，介导免疫细胞释放细胞因子，抑制肿瘤增殖、迁移和侵袭，是肿瘤免疫治疗的重要靶点。试剂盒检测范围为39 pg/ml- 2500 pg/ml，灵敏度为9.75 pg/ml。科研人员可通过开展免疫性疾病机制研究，如自身免疫模型、感染性疾病或肿瘤微环境相关实验，尤其适用于评估淋巴结、脾脏等免疫器官中趋化因子的调控规律；宽泛的线性范围特别适用于动态监测炎症模型中趋化因子的表达变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ccl19 ELISA Kit; Elc ELISA Kit; Scya19 ELISA Kit; C-C motif chemokine 19 ELISA Kit; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit; EBI1 ligand chemokine ELISA Kit; ELC ELISA Kit; Small-inducible cytokine A19 ELISA Kit
  • 缩写：
    CCL19
  • Uniprot No.：
    O70460
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/ml - 2500 pg/ml
  • 灵敏度：
    9.75 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-3β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    93-99


    1:2

    Average %

    104


    Range %

    100-107


    1:4

    Average %

    85


    Range %

    81-89


    1:8

    Average %

    98


    Range %

    94-103


  • 回收率：

    The recovery of mouse MIP-3β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    85-94


    EDTA plasma (n=4)

    108

    104-112


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2500

    2.830

    2.786

    2.808

    2.629


    1250

    2.286

    2.301

    2.294

    2.115


    625

    1.656

    1.672

    1.664

    1.485


    312.5

    1.007

    1.069

    1.038

    0.859


    156.25

    0.683

    0.698

    0.691

    0.512


    78

    0.457

    0.438

    0.448

    0.269


    39

    0.321

    0.331

    0.326

    0.147


    0

    0.178

    0.179

    0.179




  • 货期：
    3-5 working days

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