Rabbit Polyclonal IFT57 Antibody

The IFT57 antibody is a tool used to study the intraflagellar transport (IFT) complex B, which plays a critical role in the assembly and maintenance of cilia and flagella. IFT57 (also known as HIPPI or HRSL3) is a core component of the IFT-B complex, facilitating cargo transport along microtubules within cilia. This protein is evolutionarily conserved and involved in ciliogenesis, signaling pathways (e.g., Hedgehog), and cellular processes like cell division and polarization. Dysregulation of IFT57 is linked to ciliopathies, including Joubert syndrome and Bardet-Biedl syndrome, which affect multiple organs. Researchers use IFT57 antibodies in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate ciliary function, disease mechanisms, and protein interactions. The antibody typically detects endogenous IFT57 across species (human, mouse, rat) and helps validate gene knockdown/knout models. Recent studies focus on its role in cancer metastasis and neuronal development, leveraging its association with microtubule dynamics. Commercial IFT57 antibodies are often raised in rabbits or mice, with validation in ciliated cell lines or tissues. Proper controls (e.g., knockout validation) are essential due to potential cross-reactivity with related IFT proteins.