首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL2含量。CCL2是趋化因子家族的重要成员。其背景在于它能趋化单核细胞、记忆T淋巴细胞和树突状细胞等至炎症部位。研究机制方面，它主要通过与CCR2等受体结合，激活下游信号通路，参与炎症反应、肿瘤进展等多种病理生理过程。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外研究炎症性疾病动物模型、免疫细胞趋化实验或相关信号通路机制探索等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：CCL2
Uniprot No.：P28292
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.87 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of rabbit MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-99 | ||||
| EDTA plasma (n=4) | 98 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.4882.5012.4952.3227501.9851.9991.9921.8193751.4831.4711.4771.304187.50.9380.9420.9400.767940.5740.5840.5790.406470.3820.3960.3890.21623.50.2510.2530.2520.07900.1740.1720.173 | ||||||
货期：3-5 working days
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