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Mouse Eotaxin 2 ELISA Kit

  • 中文名： 小鼠嗜酸粒细胞趋化蛋白Eotaxin 2(Eotaxin 2/CCL24)ELISA Kit
货号: IPD-E07278m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠嗜酸粒细胞趋化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL24含量。CCL24，又称嗜酸性粒细胞趋化蛋白2（eotaxin-2）和骨髓祖细胞抑制因子2（MPIF-2），是一种CC趋化因子，位于人类基因组第7号染色体。CCL24通过与其唯一受体CCR3结合，主要介导特应性疾病、寄生虫感染和全身性疾病，同时促进细胞运输并调节炎症和纤维化活动。研究显示，CCL24在纤维化疾病中发挥重要作用，其表达水平与多种纤维炎症性疾病相关。试剂盒检测范围为15.6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。为探索炎症相关疾病的分子机制、评估药物干预效果或构建疾病模型提供可靠工具；适用于体外培养细胞上清、动物模型体液及组织样本的CCL24表达水平分析，满足科研人员对趋化因子网络调控研究的多元化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ccl24 ELISA Kit; Scya24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
  • 缩写：
    CCL24
  • Uniprot No.：
    Q9JKC0
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml - 1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    80-96


    1:2

    Average %

    94


    Range %

    91-105


    1:4

    Average %

    96


    Range %

    92-99


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse eotaxin 2 /CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    97

    94-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.612

    2.634

    2.623

    2.507


    500

    2.200

    2.267

    2.233

    2.117


    250

    1.453

    1.528

    1.491

    1.374


    125

    0.753

    0.729

    0.741

    0.625


    62.5

    0.349

    0.329

    0.339

    0.222


    31.2

    0.242

    0.253

    0.248

    0.131


    15.6

    0.180

    0.184

    0.182

    0.066


    0

    0.113

    0.120

    0.116




  • 货期：
    3-5 working days

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