| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FIR; FRG; PLEKHC3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human FARP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FARP2抗体的模拟参考文献示例(实际文献需通过学术数据库查询)：
1. **标题**：FARP2 regulates axonal growth in developing neurons through RhoA activation
**作者**：Yamada T, et al.
**摘要**：研究利用FARP2特异性抗体进行免疫荧光染色，发现FARP2在神经元轴突生长锥中富集，并通过激活RhoA信号通路调控细胞骨架重组，影响神经元导向。
2. **标题**：FARP2 overexpression promotes cancer cell invasion via PDZ-RhoGEF signaling
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫组化(使用抗FARP2抗体)，证实FARP2在侵袭性乳腺癌中高表达，并与PDZ-RhoGEF相互作用增强RhoC活性，促进肿瘤细胞迁移。
3. **标题**：Antibody-based blockade of FARP2 disrupts polarized membrane trafficking
**作者**：Smith KA, et al.
**摘要**：研究开发了针对FARP2胞内结构域的单克隆抗体，证实其可抑制FARP2与Arf6的结合，破坏上皮细胞极性建立过程中的膜蛋白运输。
4. **标题**：FARP2 modulates dendritic spine morphogenesis through Rac1 signaling
**作者**：Wang H, et al.
**摘要**：利用FARP2抗体进行免疫沉淀实验，发现其与Rac1特异性鸟苷酸交换因子Tiam1存在互作，调控海马神经元树突棘形态发生，影响突触可塑性。
注：以上为模拟摘要，实际文献需查阅PubMed、Web of Science等平台，结合关键词“FARP2 antibody”或“FARP2 function”获取。
FARP2 (FERM, RhoGEF, and pleckstrin domain-containing protein 2) is a multidomain protein that functions as a guanine nucleotide exchange factor (GEF) for Rho family GTPases, particularly CDC42 and Rac1. It plays a critical role in regulating cytoskeletal dynamics, cell migration, and neuronal development. The FARP2 protein contains a FERM domain (involved in membrane association), a DH/PH domain (responsible for GEF activity), and a pleckstrin homology (PH) domain that interacts with phosphoinositides, enabling spatial regulation of its activity.
FARP2 antibodies are immunological tools designed to detect and study the expression, localization, and function of FARP2 in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research has highlighted FARP2's involvement in neuronal axon guidance, synapse formation, and cancer cell invasiveness. For example, FARP2 interacts with cell surface receptors like Plexin A1 to mediate semaphorin signaling, influencing cytoskeletal rearrangements during neural circuit development. Dysregulation of FARP2 has been implicated in neurological disorders and metastatic cancers, making its antibodies valuable for mechanistic and diagnostic investigations. Validation of FARP2 antibodies includes specificity checks via knockout controls and functional assays to confirm their utility in probing FARP2-related pathways.
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