首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠正常T细胞表达和分泌因子(RANTES/CCL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL5含量。CCL5 是重要的趋化因子。其背景上，它在免疫细胞募集等免疫反应中起关键作用。研究机制方面，CCL5 能与相应受体结合，介导细胞趋化运动，还参与多种病理生理过程，如炎症、肿瘤转移等，对其研究有助于相关疾病治疗。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中炎症模型构建、免疫调节机制探究、病毒感染相关研究或药物开发中的靶点效应评估，例如通过检测实验动物血清或组织样本中RANTES/CCL5的动态变化，解析疾病发生机制或干预策略的有效性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; MuRantes ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
缩写：CCL5
Uniprot No.：P30882
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:2 | Average % | 108 | ||||
| Range % | 104-112 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 81-89 | |||||
回收率：
| The recovery of mouse RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-100 | ||||
| EDTA plasma (n=4) | 87 | 83-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1842.0092.0971.97551.8681.8981.8831.7612.51.5231.5051.5141.3921.250.8870.8980.8930.7710.6250.4750.4870.4810.3590.3120.3520.3420.3470.2250.1560.2860.2800.2830.16100.1240.1200.122 | ||||||
货期：3-5 working days
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