首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠CC趋化因子受体2(CCR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCR2含量。CCR2 是重要的趋化因子受体。其背景上，它在免疫系统中发挥关键作用，参与炎症反应、免疫细胞迁移等。研究机制方面，主要聚焦于其与配体结合后激活下游信号通路，调控细胞功能。针对它的研究有望为炎症、肿瘤等疾病治疗提供新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品广泛用于炎症机制研究、免疫细胞功能探索、疾病动物模型中趋化因子通路的调控分析，以及药物或基因干预后CCR2表达变化的定量评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccr2; Cmkbr2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; JE/FIC receptor; MCP-1 receptor; CD antigen CD192
缩写：CCR2
Uniprot No.：P51683
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse CCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 98 | 91-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6182.5422.5802.4425001.8631.8251.8441.7062501.1851.1661.1761.0381250.7920.7760.7840.64662.50.4290.4440.4370.29931.20.2990.3070.3030.16515.60.2370.2440.2410.10300.1390.1370.138 | ||||||
货期：3-5 working days
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