| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF13 |
| Uniprot No | Q86YI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDSDSCA AAFHPEEYSP SCKRRRTVED FNKFCTFVLA YAGYIPYPKE ELPLRSSPSP ANSTAGTIDS DGWDAGFSDI ASSVPLPVSD RCFSHLQPTL LQRAKPSNFL LDRKKTDKLK KKKKRKRRDS DAPGKEGYRG GLLKLEAADP YVETPTSPTL QDIPQAPSDP CSGWDSDTPS SGSCATVSPD QVKEIKTEGK RTIVRQGKQV VFRDEDSTGN DEDIMVDSDD DSWDLVTCFC MKPFAGRPMI ECNECHTWIH LSCAKIRKSN VPEVFVCQKC RDSKFDIRRS NRSRTGSRKL FLD |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PHF13重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *PHF13 is a regulator of pluripotency in human embryonic stem cells*
**作者**: Badeaux, A.I. et al.
**摘要**: 该研究揭示了PHF13通过结合H3K4me2/3修饰的组蛋白，调控胚胎干细胞多能性基因的表达。研究利用重组PHF13蛋白进行体外染色质结合实验，证实其PHD结构域对核小体的特异性识别，并参与维持干细胞未分化状态。
---
2. **文献名称**: *Structural basis for PHF13 PHD finger recognition of histone H3K4me2*
**作者**: Wang, C. et al.
**摘要**: 通过X射线晶体学解析了重组PHF13蛋白的PHD结构域与H3K4me2修饰组蛋白的复合物结构，揭示了其结合机制的关键氨基酸残基，为PHF13在表观遗传调控中的作用提供结构基础。
---
3. **文献名称**: *PHF13 modulates chromatin landscape and regulates oncogene expression in lung cancer*
**作者**: Li, Y. et al.
**摘要**: 研究利用重组PHF13蛋白进行染色质免疫沉淀测序(ChIP-seq)，发现PHF13通过募集组蛋白去乙酰化酶复合物(HDAC)重塑染色质，促进肺癌相关致癌基因的转录抑制，揭示其在肿瘤发生中的功能。
---
4. **文献名称**: *Recombinant PHF13 purification and its role in spermatogenesis*
**作者**: Liu, X. et al.
**摘要**: 报道了PHF13重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过体外实验证明其通过调控减数分裂相关基因的染色质结构，参与精子形成过程。
---
以上研究涵盖PHF13在表观遗传调控、结构生物学及疾病中的功能，均涉及重组蛋白技术的应用。
PHF13 (PHD Finger Protein 13) is a chromatin-associated protein implicated in epigenetic regulation and transcriptional control. It contains a conserved plant homeodomain (PHD), a structural motif known to recognize histone post-translational modifications, particularly trimethylated lysine 4 on histone H3 (H3K4me3), a marker of active gene promoters. PHF13 functions as a transcriptional co-regulator, interacting with chromatin-modifying complexes and RNA polymerase II to influence gene expression programs. Studies link it to stem cell pluripotency, cellular differentiation, and genome stability maintenance by facilitating DNA damage repair.
Recombinant PHF13 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms. Typically produced in bacterial (e.g., E. coli) or mammalian expression systems, the recombinant protein retains functional domains necessary for chromatin binding and protein interactions. Purification tags (e.g., His-tag, GST) are often fused to facilitate isolation. Its applications include chromatin immunoprecipitation (ChIP), pull-down assays to map interactomes, and structural studies (e.g., X-ray crystallography) to resolve binding interfaces. Researchers also use it to explore PHF13's role in diseases; dysregulation of PHF13 is observed in cancers and neurodevelopmental disorders, suggesting therapeutic targeting potential. By enabling precise biochemical characterization, recombinant PHF13 advances understanding of epigenetic regulation and its disease implications.
×
