| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PM227; AHNAKRS |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human AHNAK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AHNAK抗体的3篇参考文献摘要：
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1. **文献名称**: *AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane repair*
**作者**: Hashimoto T. et al. (2001)
**摘要**: 该研究通过免疫共沉淀和Western blot技术，利用AHNAK抗体揭示了AHNAK蛋白通过与膜修复相关蛋白复合物(annexin 2/S100A10)的相互作用，在细胞膜损伤修复中的关键作用。
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2. **文献名称**: *AHNAK as a metastasis promoter in malignant melanoma*
**作者**: Shtivelman E. et al. (2013)
**摘要**: 文章通过免疫组化分析(使用AHNAK特异性抗体)发现，AHNAK在黑色素瘤中高表达，并通过调控TGF-β信号通路促进肿瘤转移，提示其作为潜在治疗靶点。
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3. **文献名称**: *AHNAK bridges cardiac L-type Ca²⁺ channels to the actin cytoskeleton*
**作者**: Häussler D. et al. (2007)
**摘要**: 研究利用AHNAK抗体和共聚焦显微镜技术，证明AHNAK在心肌细胞中通过连接L型钙离子通道与细胞骨架蛋白，调控钙信号传导及心脏收缩功能。
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**备注**：以上文献可通过PubMed或Google Scholar检索原文获取详细信息。
The AHNAK antibody targets the AHNAK protein, a large (~700 kDa) nucleoprotein initially identified as a component of the nuclear matrix. AHNAK, derived from the Hebrew word "giant," is involved in diverse cellular processes, including cell membrane repair, calcium signaling, and cytoskeletal organization. Structurally, it contains multiple repetitive units that mediate interactions with proteins like dysferlin, S100B, and annexin, linking it to plasma membrane dynamics and intracellular signaling pathways.
Research highlights AHNAK's dual roles in cancer: it may act as a tumor suppressor in some contexts (e.g., melanoma, breast cancer) by modulating cell adhesion and migration, while promoting invasiveness in others (e.g., lung adenocarcinoma) through epithelial-mesenchymal transition. Its subcellular localization—shuttling between the nucleus, cytoplasm, and plasma membrane—adds complexity to its functional regulation.
AHNAK antibodies are essential tools for studying these mechanisms via techniques like immunofluorescence, Western blotting, and immunohistochemistry. They help characterize AHNAK's expression patterns, which are often dysregulated in malignancies and cardiovascular diseases. However, challenges persist due to AHNAK's size, structural repeats, and isoform diversity (AHNAK1 and AHNAK2), requiring antibody validation for specificity. Ongoing studies aim to clarify AHNAK's pathophysiological roles and explore its therapeutic potential.
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