| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHPT1 |
| Uniprot No | Q9NRX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAVADLALIPDVDIDSDGVFKYVLIRVHSA PRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRIS HQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PHPT1重组蛋白的示例性参考文献(内容为模拟概括，仅供参考)：
1. **文献名称**：*Expression and Purification of Recombinant Human PHPT1 in Escherichia coli*
**作者**：Zhang Y, et al.
**摘要**：报道了利用大肠杆菌系统成功表达并纯化重组人源PHPT1蛋白，验证其磷酸酶活性，为功能研究提供基础材料。
2. **文献名称**：*Structural Insights into PHPT1 Catalytic Mechanism by X-ray Crystallography*
**作者**：Liu X, et al.
**摘要**：通过X射线晶体学解析PHPT1的三维结构，揭示了其活性位点关键氨基酸残基，阐明了底物结合与催化机制。
3. **文献名称**：*PHPT1 Regulates Cellular Signaling via Dephosphorylation of Histidine Phosphorylated Proteins*
**作者**：Chen L, et al.
**摘要**：研究证明PHPT1特异性去磷酸化组氨酸磷酸化蛋白，参与调控MAPK等信号通路，影响细胞增殖与凋亡。
4. **文献名称**：*Dysregulation of PHPT1 in Cancer and Its Role in Tumor Metastasis*
**作者**：Wang H, et al.
**摘要**：发现PHPT1在多种癌症中异常高表达，通过激活EMT通路促进肿瘤转移，提示其作为潜在治疗靶点。
**备注**：以上文献信息为示例，实际引用时请通过PubMed、Web of Science等数据库检索真实文献。
**Background of PHPT1 Recombinant Protein**
PHPT1 (phosphohistidine phosphatase 1), also known as LHPP or histidine phosphatase superfamily member 1. is a conserved enzyme that specifically dephosphorylates phosphohistidine residues—a rare but critical post-translational modification involved in cellular signaling. Unlike serine/threonine or tyrosine phosphorylation, histidine phosphorylation is less studied, and PHPT1 is one of the few phosphatases identified to regulate this modification. It plays a role in modulating metabolic pathways, signal transduction (e.g., Ras/MAPK, PI3K/Akt), and energy homeostasis by reversing histidine phosphorylation in proteins such as ATP-citrate lyase (ACLY) and G-proteins.
Recombinant PHPT1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for functional studies. Its structure, resolved via crystallography, reveals a conserved α/β-hydrolase fold with a catalytic triad critical for phosphatase activity. Researchers utilize PHPT1 recombinant protein to investigate its enzymatic mechanisms, substrate specificity, and interactions with signaling molecules.
PHPT1 has gained attention for its potential roles in disease. Studies link its dysregulation to cancers (e.g., hepatocellular carcinoma, glioblastoma), where it may act as a tumor suppressor, and metabolic disorders like diabetes. Recombinant PHPT1 is also used in drug discovery to screen inhibitors or activators targeting histidine phosphorylation pathways. Ongoing research aims to clarify its physiological relevance and therapeutic potential, making PHPT1 a protein of growing interest in both basic and applied biomedical sciences.
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